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Status |
Public on Jan 15, 2013 |
Title |
Genome-wide identification of Ikaros binding sites in primary pre-B cells |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Ikaros DNA binding proteins are important regulators of haematopoiesis and genetic deletion of Ikaros results in severe developmental disturbances, including delayed thymocyte differentiation and an early and complete block in B cell development. Although Ikaros ChIP-seq data are available for mouse thymocytes and human haematopoietic progenitors, it has not been achieved in B cell progenitors. The goal of this study was to identify Ikaros binding sites in pre-B cells to define Ikaros target genes which could explain the essential role of Ikaros proteins in B cell differentiation.
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Overall design |
We carried out chromatin immunoprecipitation and high throughput sequencing (ChIP-seq) with antibodies to the C-terminus of endogenous Ikaros in primary pre-B cells isolated from mouse fetal livers.
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Contributor(s) |
Ferreirós Vidal I, Terry A, Dharmalingam G, Merkenschlager M |
Citation(s) |
23303821 |
Submission date |
Nov 21, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Gopuraja Dharmalingam |
Organization name |
MRC London Institute of Medical Sciences
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Department |
Epigenetics section
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Street address |
Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
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City |
London |
ZIP/Postal code |
W12 0NN |
Country |
United Kingdom |
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Platforms (1) |
GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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Samples (4)
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GSM1040573 |
endogenous Ikaros_rep1, lanes 1 and 2 [primary pre-B cells] |
GSM1040574 |
endogenous Ikaros_rep2, lanes 1 and 2 [primary pre-B cells] |
GSM1040575 |
input DNA_rep1 [primary pre-B cells] |
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This SubSeries is part of SuperSeries: |
GSE38200 |
Ikaros target genes in the mouse pre-B cell line B3 |
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Relations |
BioProject |
PRJNA182016 |
SRA |
SRP017277 |