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Series GSE42591 Query DataSets for GSE42591
Status Public on Jul 01, 2013
Title Expression data from fresh and cultured islets at different glucose concentrations
Organism Mus musculus
Experiment type Expression profiling by array
Summary β-cell identity is determined by tightly regulated transcriptional networks that are modulated by extracellular cues, thereby ensuring β-cell adaptation to the organism’s insulin demands. We have observed in pancreatic islets that stimulatory glucose concentrations induced a gene profile that was similar to that of freshly isolated islets, indicating that glucose-elicited cues are involved in maintaining β-cell identity. Low glucose induces the expression of ubiquitous genes involved in stress responses, nutrient sensing, and organelle biogenesis. By contrast, stimulatory glucose concentrations activate genes with a more restricted expression pattern (β- and neuronal- cell identity). Consistently, glucose-induced genes are globally reduced in islets deficient with Hnf1a (MODY3), characterized by a deficient glucose metabolism. Of interest, a cell cycle gene module was the most enriched among the variable genes between intermediate and stimulatory glucose concentrations. Glucose regulation of the islet transcriptome was unexpectedly broadly maintained in islets from aged mice. However, the cell cycle gene module is selectively lost in old islets and the glucose activation of this module is not recovered even in the absence of the cell cycle inhibitor p16.
We used microarrays to detail the global programme of gene expression regulated by glucose in young and aged pancreatic islets as well as freshly-isolated islets.
 
Overall design Pancreatic islets from young and old mice were isolated and cultured at different glucose concentrations for RNA extraction and hybridization on Affymetrix microarrays. Islets were cultured at 3mM (G3), 5.5mM (G5), 11mM (G11) and 16mM (G16). Freshly-isolated islets (F) were also processed for RNA extraction . We also assessed the dynamic glucose regulation of gene expression at different time-points after an overnight at 3mM (T0): after 1h at 11mM (T1) and after 4h (T4).
 
Contributor(s) Servitja J, Moreno-Asso A
Citation(s) 23685457
Submission date Nov 28, 2012
Last update date Feb 11, 2019
Contact name Joan Marc Servitja
E-mail(s) servitja@clinic.ub.es
Organization name IDIBAPS
Street address Rosselló, 153
City Barcelona
State/province Catalonia
ZIP/Postal code 08036
Country Spain
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (26)
GSM1045554 old islets at G3, biological rep1
GSM1045555 old islets at G3, biological rep2
GSM1045556 old islets at G5, biological rep1
Relations
BioProject PRJNA182382

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Supplementary file Size Download File type/resource
GSE42591_RAW.tar 131.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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