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| Status |
Public on Nov 29, 2012 |
| Title |
An Organ Culture System to Model Early Degenerative Changes of the Intervertebral Disc: II Profiling Global Gene Expression Changes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To mimic a degenerative insult, rat intervertebral discs were cultured in the presence of TNF-alpha, IL-1beta and serum limiting conditions.
Gene expression analysis was performed using a microarray to identify differential gene expression between experimental and control groups.
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| Overall design |
The discs were collected and the annulus fibrosus (AF) was separated from the nucleus pulposus (NP). Total RNA from NP was used for RNA extraction and hybridization on Affymetrix microarrays: Control group - Ctr-1, Ctr-2, Ctr-3, Ctr-4 and Experimental group-Exp-1, Exp-2, Exp-3, Exp-4.
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| Contributor(s) |
Markova DZ, Kepler CK, Addya S, Murray HB, Vaccaro AR, Shapiro IM, Anderson DG, Albert TJ, Risbud MV |
| Citation(s) |
28940640, 24171898 |
| Submission date |
Nov 29, 2012 |
| Last update date |
Feb 03, 2020 |
| Contact name |
Dessislava Z Markova |
| E-mail(s) |
Dessislava.Markova@Jefferson.edu
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| Phone |
2159557655
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| Fax |
2159559773
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| Organization name |
Thomas Jefferson University
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| Department |
Orthopaedic Surgery
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| Lab |
508
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| Street address |
1015 Walnut
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA182353 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42611_RAW.tar |
28.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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