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| Status |
Public on Aug 12, 2013 |
| Title |
Effect of cigarette smoke on gene expression in mammary epithelial cells [methylation] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
Recent epidemiological studies demonstrate that both active and involuntary exposure to tobacco smoke increases the risk of breast cancer. Little is known, however, about the molecular mechanisms by which tobacco smoke contributes to breast carcinogenesis. To investigate these mechanisms we have analyzed gene expression and methylation in MCF 10A mammary epithelial cells chronically exposed to aqueous cigarette smoke extract (CSE).
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| Overall design |
CSE was prepared weekly and added to the cell cultures at a concentration equivalent to 0.001 cigarettes/ml for 21 weeks. Mock-treated samples were prepared in parallel.
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| Contributor(s) |
Zahnow CA, Di Cello F |
| Citation(s) |
23919753 |
| Submission date |
Nov 29, 2012 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Francescopaolo Di Cello |
| E-mail(s) |
fdicello@hotmail.com
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| Organization name |
The Johns Hopkin University School of Medicine
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| Street address |
1650 Orleans Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
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| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE42668 |
Effect of cigarette smoke on gene expression in mammary epithelial cells |
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| Relations |
| BioProject |
PRJNA182597 |
