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| Status |
Public on Aug 01, 2013 |
| Title |
Expression data from Tet2-hypomorph (knockdown) and/or Ezh2-null Lineage-Sca-1+c-Kit+ (LSK) cells and granulocyte-macrophage progenitors (GMPs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
PcG proteins form the polycomb repressive complexes (PRC) 1 and 2, functioning as transcriptional repressors through histone modifications. They have been implicated in the maintenance of self-renewing somatic and cancer stem cells. PcG genes have been characterized as tumor suppressor genes as exemplified by somatic inactivating mutations of EZH2, a gene encoding histone methyltransferase in PRC2, in myeloid malignancy. Mice deficient for Tet2 have been reported to recapitulate some aspects of myeloid malignancies. We evaluated the Ezh2-deficient mice and also tested the impact of concurrent depletion of Ezh2 and Tet2 on hematopoiesis.
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| |
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| Overall design |
Purified LSK cells and GMPs from BM of recipient mice repopulated with wild-type, Tet2KD/KD, Ezh2-/- and Tet2KD/KDEzh2-/- fetal liver cells were subjected to RNA extraction and hybridization on Agilent microarrays.
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| |
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| Contributor(s) |
Iwama A, Muto T |
| Citation(s) |
24218139 |
| Submission date |
Dec 02, 2012 |
| Last update date |
Jul 19, 2017 |
| Contact name |
ATSUSHI IWAMA |
| E-mail(s) |
aiwama@faculty.chiba-u.jp
|
| Phone |
81-43-226-2187
|
| Fax |
81-43-226-2191
|
| Organization name |
Graduate School of Medicine, Chiba University
|
| Department |
Department of Cellular and Molecular Medicine
|
| Street address |
1-8-1 Inohana, Chuo-ku
|
| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA182595 |
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