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| Status |
Public on Dec 05, 2012 |
| Title |
Effect of synbindin on the gene expression profile of gastric cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In order to determine the effect of synbindin on the gene expression profile of gastric cancer cells, MGC-803 cells were infected with adenoviruses carrying either synbindin or GFP. The differentially expressed genes caused by synbindin ectopic expression suggests the underlying mechanism for synbindin-induced cell proliferation.
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| Overall design |
Total RNA from each sample was amplified and transcribed into fluorescent cRNA using Agilent's Quick Amp Labeling protocol (version 5.7, Agilent Technologies). The labeled cRNAs were hybridized onto the Whole Human Genome Oligo Microarray (4 x 44K, Agilent Technologies). After washing, the arrays were scanned by the Agilent Scanner G2505C. 3 replicates per treatment (GFP adenovirus, synbindin adenovirus).
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| Contributor(s) |
Kong X, Xu J, Fang JY |
| Citation(s) |
24104608 |
| Submission date |
Dec 04, 2012 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Jie Xu |
| E-mail(s) |
xujieletter@gmail.com
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| Organization name |
Shanghai Institute for Digestive Diseases
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| Department |
Shanghai Renji Hospital
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| Lab |
State Key Laboratory for Oncogenes and Related Genes
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| Street address |
Shandong Rd 145
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| City |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA182966 |
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