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Series GSE42839 Query DataSets for GSE42839
Status Public on Dec 11, 2012
Title Expression changes in MEL cells upon differentiation and Ldb1 knockdown
Organism Mus musculus
Experiment type Expression profiling by array
Summary Carbonic anhydrase 1 (Car1), an early specific marker of the erythroid differentiation, has been used to distinguish fetal and adult erythroid cells since its production closely follows the γ- to β-globin transition, but the molecular mechanism underlying transcriptional regulation of Car1 is unclear. Here, we show that Car1 mRNA decreases significantly when erythroid differentiation is induced in MEL cells. The Ldb1 protein complex including GATA1/SCL/LMO2 binds to the Car1 promoter in uninduced cells and reduced enrichment of the complex during differentiation correlates with loss of Car1 expression. Knockdown of Ldb1 results in a reduction of Ser2 phosphorylated RNA Pol II and Cdk9 at the Car1 promoter region, suggesting that Ldb1 is required for recruitment of Pol II as well as the transcription regulator P-TEFb to enhance elongation of Car1 transcripts. Taken together, these data show that Ldb1 forms a regulatory complex to maintain Car1 expression in erythroid cells.
 
Overall design Expression analysis of induced and uninduced MEL cells after control or Ldb1 shRNA knockdown.
 
Contributor(s) Dale RK, Dean A
Citation(s) 22609543
Submission date Dec 11, 2012
Last update date Dec 13, 2012
Contact name Ryan Dale
E-mail(s) dalerr@nih.gov
Organization name National Institutes of Health
Department National Institute of Child Health and Human Development
Lab Bioinformatics and Scientific Programming Core
Street address Rm 10D39, 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL16368 [Mouse4302_Mm_ENTREZG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 16]
Samples (10)
GSM1051215 MEL_control_induced_rep1
GSM1051216 MEL_control_induced_rep2
GSM1051217 MEL_control_induced_rep3
Relations
BioProject PRJNA183613

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Supplementary file Size Download File type/resource
GSE42839_RAW.tar 78.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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