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| Status |
Public on Apr 01, 2013 |
| Title |
Marker gene discovery for Staphylococcus epidermidis infection in zebrafish embryos (RNA-Seq) |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We use the zebrafish embryo model to study the innate immune response against Staphylococcus epidermidis. Therefore, we injected S. epidermidis into the yolk at 2 hpf and took samples at 5 days post injection.
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| Overall design |
This deep sequence study was designed to determine the gene expression profile by Staphylococcus epidermidis infection. RNA was isolated from embryos at 5 days post injection. Wildtypes zebrafish embryos were micro-injected into the yolk (2hpf) with 20 CFU of S. epidermdis O-47 mCherry bacteria suspended in PVP (Polyvinylpyrrolidone), or Non-injected as a control. After injections embryos were transferred into fresh egg water and incubated at 28°C. At 5 days post injection 100-200 embryos per group were snap-frozen in liquid nitrogen, and total RNA was isolated using TRIZOL reagent.
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| Contributor(s) |
Veneman WJ, Spaink HP, Meijer AH |
| Citation(s) |
23586901, 25503064 |
| Submission date |
Dec 11, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Wouter Veneman |
| E-mail(s) |
w.j.veneman@biology.leidenuniv.nl
|
| Organization name |
Leiden University
|
| Department |
Institute Biology Leiden
|
| Lab |
Molecular Cell Biology
|
| Street address |
Einsteinweg 55
|
| City |
Leiden |
| State/province |
Zuid-Holland |
| ZIP/Postal code |
2333CC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE42847 |
Marker gene discovery for Staphylococcus epidermidis infection in zebrafish embryos |
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| Relations |
| BioProject |
PRJNA183633 |
| SRA |
SRP017511 |
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