 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 05, 2007 |
| Title |
Making a predictive heart failure expression profile in Ren2 rat left ventricles |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
The intercalated disc of cardiac myocytes is emerging as a crucial structure in the heart. Loss of intercalated disc proteins like N-cadherin causes lethal cardiac abnormalities, mutations in intercalated disc proteins cause human cardiomyopathy. A comprehensive screen for novel mechanisms in failing hearts demonstrated that expression of the lysosomal integral membrane protein-2 (LIMP-2) is increased in cardiac hypertrophy and heart failure in both rat and human myocardium. Complete loss of LIMP-2 in genetically engineered mice did not affect cardiac development; however these LIMP-2 null mice failed to mount a hypertrophic response to increased blood pressure but developed cardiomyopathy. Disturbed cadherin localization in these hearts suggested that LIMP-2 has important functions outside lysosomes. Indeed, we also find LIMP-2 in the intercalated disc, where it associates with cadherin. RNAi-mediated knockdown of LIMP-2 decreases the binding of phosphorylated b-catenin to cadherin, while overexpression of LIMP-2 has the opposite effect. Taken together, our data show that lysosomal integrated membrane protein-2 is crucial to mount the adaptive hypertrophic response to cardiac loading. We demonstrate a novel role for LIMP-2 as an important mediator of the intercalated disc.
Keywords: heart failure, comparison
|
| |
|
| Overall design |
overall design:
3 groups of rats, 1 sample per rat:
- compensated = Ren2 rat, hypertensive, no heart failure (N=6)
- failure = Ren2 rat, hypertensive, no heart failure (N=4)
- SD = control group, non-hypertensive (N=4)
|
| |
|
| Contributor(s) |
van Erk A, Schroen B, Pinto Y |
| Citation(s) |
17485520 |
| Submission date |
Feb 21, 2006 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Arie van Erk |
| E-mail(s) |
arie.vanerk@bigcat.unimaas.nl
|
| Phone |
+31 (043) 3882913
|
| Organization name |
University Maastricht
|
| Department |
BiGCaT Bioinformatics
|
| Lab |
Experimental and Molecular Cardiology
|
| Street address |
PO Box 616
|
| City |
Maastricht |
| ZIP/Postal code |
6200MD |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
|
| Samples (14)
|
| GSM180061 |
Ren2, compensated, rat 4 |
| GSM180062 |
Ren2, compensated, rat 5 |
| GSM180065 |
Ren2, compensated, rat 6 |
| GSM180068 |
Ren2, failure, rat 7 |
| GSM180069 |
Ren2, failure, rat 8 |
| GSM180073 |
Ren2, failure, rat 9 |
| GSM180075 |
Ren2, failure, rat 10 |
| GSM180079 |
SD, control, rat 11 |
| GSM180082 |
SD, control, rat 12 |
| GSM180085 |
SD, control, rat 13 |
| GSM180089 |
SD, control, rat 14 |
|
| Relations |
| BioProject |
PRJNA94779 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4286_RAW.tar |
174.8 Mb |
(http)(custom) |
TAR (of CEL, CHP, EXP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...