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Series GSE42889 Query DataSets for GSE42889
Status Public on Dec 13, 2012
Title Expression data from adult mouse ovaries exposed to cigarette smoke in vivo
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mammalian females are born with a finite number of non-renewing primordial follicles, the majority of which remain in a quiescent state for many years. Due to their non-renewing nature, these “resting” oocytes are particularly vulnerable to xenobiotic insult, resulting in premature ovarian senescence and the formation of dysfunctional oocytes. In this study we characterised the mechanisms behind cigarette smoke induced ovotoxicity, which is characterised by primordial follicle depletion. C57BL/6 5 week old female mice were exposed to cigarette smoke five times per week, for 12-18 weeks using a custom-designed and purpose-built nose-only, directed flow inhalation and smoke-exposure system. This was done in the hopes of gaining a better understanding of the mechanisms underpinning cigarette smoke induced ovotoxicity.
 
Overall design C57BL/6 5 week old female mice were exposed to cigarette smoke (twelve 3R4F reference cigarettes (University of Kentucky, USA) twice/day, 2.7 mg particulate matter) five times per week, for 12-18 weeks using a custom-designed and purpose-built nose-only, directed flow inhalation and smoke-exposure system. Their ovaries were then collected for RNA extraction and hybridization on an Illumina Sentrix Mouse ref-8 v2 Beadchip
 
Contributor(s) Sobinoff AP, McLaughlin EA
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Submission date Dec 12, 2012
Last update date Jun 14, 2018
Contact name Alexander Peter Sobinoff
E-mail(s) c3045119@uon.edu.au
Organization name University of Newcastle Australia
Department Science and IT
Lab Reproductive Science Group
Street address University Drive
City Newcastle
State/province NSW
ZIP/Postal code 2308
Country Australia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM1052767 Control rep 1
GSM1052768 Control rep 2
GSM1052769 Control rep 3
Relations
BioProject PRJNA183706

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42889_Comparison1_Ovary.xlsx.gz 12.8 Mb (ftp)(http) XLSX
GSE42889_RAW.tar 3.1 Mb (http)(custom) TAR
GSE42889_non-normalized.txt.gz 474.3 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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