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Series GSE4296 Query DataSets for GSE4296
Status Public on May 01, 2006
Title Gene expression analysis of testicular DLBCL: ABC or GCB subtype?
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Diffuse large B cell lymphomas (DLBCL) constitute a heterogeneous group of lymphomas in which germinal center B cell-like and activated B cell-like subtypes can be discerned based on pathology, clinical presentation and gene expression patterns. Testicular DLBCL form an immune-privileged site-related subgroup of DLBCL with an unfavorable prognosis. We used cDNA microarray analysis, immunohistochemistry for CD10, Bcl6 and MUM1, and somatic hypermutation analysis of the immunoglobulin heavy chain gene rearrangements to determine the subtype of primary testicular DLBCL. Immunohistochemistry revealed 14/22 testicular DLBCL with an activated B cell-like immunophenotype and 8/22 with an ambiguous immunophenotype co-expressing CD10 and high levels of MUM1. cDNA microarray analysis of these 22 and 4 additional cases showed a uniform activated B cell-like gene expression pattern in both immunophenotypes. Somatic hypermutation analysis showed a very high mutation load in 7 cases tested, but intraclonal heterogeneity was found at low level in only one of these cases. We conclude that primary testicular DLBCL have uniform activated B cell-like subtype characteristics despite a number of cases showing an ambiguous immunophenotype.
Keywords: Gene expression
 
Overall design Twenty-six primary testicular DLBCL and 10 primary nodal DLBCL were analyzed using an 18k cDNA microarray. Hierarchical clustering was done based on a set of 21 genes and a set of 201 genes, both discriminative for ABC/GCB subtypes, to classify cases. Gene expression profiles were compared between testicular DLBCL of ambiguous and activated B cell-like immunophenotype.

Immunophenotype classification of Nodal DLBCL and Testicular DLBCL samples: see Table 1 in PMID 16823896.
 
Contributor(s) Booman M, Douwes J, Glas AM, de Jong D, Schuuring E, Kluin PM
Citation(s) 16823896
Submission date Feb 24, 2006
Last update date Oct 03, 2012
Contact name Marije Booman
E-mail(s) m.booman@path.umcg.nl
Organization name University Medical Center Groningen
Department Pathology
Street address P.O. Box 30001
City Groningen
ZIP/Postal code 9700 RB
Country Netherlands
 
Platforms (1)
GPL3408 NKI/CMF Human 18k cDNA spotted microarray
Samples (36)
GSM94779 4N
GSM94782 3N
GSM94786 6N
Relations
BioProject PRJNA94675

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4296_Imagene_filename_explanation.txt 374 b (ftp)(http) TXT
GSE4296_RAW.tar 267.7 Mb (http)(custom) TAR (of IMAGENE)
Raw data provided as supplementary file

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