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GEO help: Mouse over screen elements for information. |
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Status |
Public on Dec 10, 2013 |
Title |
MicroRNA transcriptional regulation in lung cancer |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Squamous cell lung cancer (SCC) and adenocarcinoma are the most common subtypes of the lung tumours. The search for cancer-directed treatments has increased the need for understanding molecular features of either histological subtype. The aim of this study was to identify transcriptional regulation differences due to miRNA expression profiles between SCC and adenocarcinoma. For this propose, a total of 44 patients were evaluated to assess the correlation between the miRNA and messenger RNA (mRNA) expression levels. Total RNA were isolated, amplified, labeled and hybridized on Agilent human whole genome V2 22 K microarray chip. qRT-PCR was conducted to validate our microarray data. MicroRNA expression was detected and quantified using the TaqMan Low Density Arrays. Predicted miRNA-mRNA interactions were taken from miRanda, miRWalk and TargetScan. After processing data, changes in 56 mRNAs as well as in 9 miRNAs were detected between SCC and adenocarcinoma. Nearly 20% of overall deregulated genes were targeted by at least one of the 9 miRNAs (miR-149, miR-205, miR-375, miR-378, miR-422a, miR-483-5p, miR-494, miR-601 and miR-708) differentially expressed between SCC and adenocarcinoma. Genes predicted (CEACAM6, CGN, CLDN3, ABCC3, MLPH, ACSL5, TMEM45B, MUC1) to be targeted by several miRNAs were individually validated by qRT-PCR. We found genes involved in tight junctions and other involved in resistance to anticancer agents. These genes were reliable biomarkers, with high sensitivity and specificity, to detect differences between the two most common histological subtypes of lung cancer. In conclusion, our data demonstrate that the transcriptional regulation differences through miRNA expression play an important role in key hallmarks of non-small cell lung cancer. Identification of new molecular markers in lung carcinoma
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Overall design |
44 tumour samples following surgical resection for clinical early stage NSCLC (SCC and adenocarcinoma) This series represents the mRNA profiling only (not miRNA). The related miRNA data are in Series GSE43000.
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Contributor(s) |
Molina-Pinelo S, Pozo G, Pastor M, Hergueta M, Moreno-Bueno G, García-Carbonero R, Nogal A, Suárez R, Salinas A, Pozo-Rodríguez F, Lopez-Rios F, Agulló-Ortuño M, Carnero A, Paz-Ares L |
Citation(s) |
24625834 |
Submission date |
Dec 18, 2012 |
Last update date |
Aug 22, 2019 |
Contact name |
Gema Moreno-Bueno |
E-mail(s) |
gmoreno@iib.uam.es
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Phone |
+34914978974
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Organization name |
IIB
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Department |
Biochemistry
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Lab |
1.13
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Street address |
Arturo Duperier
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City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28029 |
Country |
Spain |
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Platforms (1) |
GPL1708 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version) |
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Samples (44)
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Relations |
BioProject |
PRJNA184033 |
Supplementary file |
Size |
Download |
File type/resource |
GSE42998_RAW.tar |
508.2 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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