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Series GSE42998 Query DataSets for GSE42998
Status Public on Dec 10, 2013
Title MicroRNA transcriptional regulation in lung cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Squamous cell lung cancer (SCC) and adenocarcinoma are the most common subtypes of the lung tumours. The search for cancer-directed treatments has increased the need for understanding molecular features of either histological subtype. The aim of this study was to identify transcriptional regulation differences due to miRNA expression profiles between SCC and adenocarcinoma. For this propose, a total of 44 patients were evaluated to assess the correlation between the miRNA and messenger RNA (mRNA) expression levels. Total RNA were isolated, amplified, labeled and hybridized on Agilent human whole genome V2 22 K microarray chip. qRT-PCR was conducted to validate our microarray data. MicroRNA expression was detected and quantified using the TaqMan Low Density Arrays. Predicted miRNA-mRNA interactions were taken from miRanda, miRWalk and TargetScan. After processing data, changes in 56 mRNAs as well as in 9 miRNAs were detected between SCC and adenocarcinoma. Nearly 20% of overall deregulated genes were targeted by at least one of the 9 miRNAs (miR-149, miR-205, miR-375, miR-378, miR-422a, miR-483-5p, miR-494, miR-601 and miR-708) differentially expressed between SCC and adenocarcinoma. Genes predicted (CEACAM6, CGN, CLDN3, ABCC3, MLPH, ACSL5, TMEM45B, MUC1) to be targeted by several miRNAs were individually validated by qRT-PCR. We found genes involved in tight junctions and other involved in resistance to anticancer agents. These genes were reliable biomarkers, with high sensitivity and specificity, to detect differences between the two most common histological subtypes of lung cancer. In conclusion, our data demonstrate that the transcriptional regulation differences through miRNA expression play an important role in key hallmarks of non-small cell lung cancer.
Identification of new molecular markers in lung carcinoma
 
Overall design 44 tumour samples following surgical resection for clinical early stage NSCLC (SCC and adenocarcinoma)
This series represents the mRNA profiling only (not miRNA). The related miRNA data are in Series GSE43000.
 
Contributor(s) Molina-Pinelo S, Pozo G, Pastor M, Hergueta M, Moreno-Bueno G, García-Carbonero R, Nogal A, Suárez R, Salinas A, Pozo-Rodríguez F, Lopez-Rios F, Agulló-Ortuño M, Carnero A, Paz-Ares L
Citation(s) 24625834
Submission date Dec 18, 2012
Last update date Aug 22, 2019
Contact name Gema Moreno-Bueno
E-mail(s) gmoreno@iib.uam.es
Phone +34914978974
Organization name IIB
Department Biochemistry
Lab 1.13
Street address Arturo Duperier
City Madrid
State/province Madrid
ZIP/Postal code 28029
Country Spain
 
Platforms (1)
GPL1708 Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version)
Samples (44)
GSM1054641 7060047
GSM1054642 7060270
GSM1054643 7050866
Relations
BioProject PRJNA184033

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42998_RAW.tar 508.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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