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| Status |
Public on Oct 01, 2013 |
| Title |
Gene expression profile upon IL-22 stimulation, forced expression of Hes1, or IL-22 stimulation under forced expression of Hes1, in human colon carcinoma-derived LS174T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A human colon carcinoma-derived cell line LS174T was modified to overexpress Hes1, a bHLH-type transcription factor, upon doxycycline addition (designated as LS174T-tetON-Hes1 cells), using the T-rex system (Invitrogen). We have previously shown that these cells can overexpress Hes1 under the control of CMV promoter (Zheng et al, Inflamm Bowel Dis, 17;2251-2260, 2011), and the amont of the overexpressed Hes1 protein reaches to the maximal level in early as 3 hours from doxycycline addition (100ng/ml), which persists for up to 24 hours. In the present experiment, LS174T-tetON-Hes1 cells were treated with recombinant human IL-22 (20ng/ml) alone, doxycycline alone (100ng/ml), co-treated by both IL-22 and doxycycline, or left untreated (Control) for 24 hours, and subjected for analysis.
Experiment was done using a modified sub-line of LS174T cell (LS174T-tetON-Hes1 cells), in which overexpression of Hes1 can be induced by a Doxycycline dependent manner.
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| Overall design |
Four-condition experiment, Control vs. IL-22 stimulation, Control vs Doxycycline addition (Hes1 overexpression) and Control vs IL22 stimulation+Doxycycline addition (Hes1 overexpression). Biological replicates: One for each condition, independently grown and harvested. One replicate per array.
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| Contributor(s) |
Okamoto R, Murano T |
| Citation(s) |
24342613 |
| Submission date |
Dec 19, 2012 |
| Last update date |
Aug 02, 2018 |
| Contact name |
Ryuichi Okamoto |
| E-mail(s) |
rokamoto.gast@tmd.ac.jp
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| Organization name |
Tokyo Medical and Dental University
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| Street address |
Tokyo Medical and Dental University
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| City |
Tokyo |
| State/province |
Please Select |
| ZIP/Postal code |
113-8519 |
| Country |
Japan |
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| Platforms (1) |
| GPL13915 |
3D-Gene Human Oligo chip 25k V2.1 |
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| Samples (3) |
| GSM1055656 |
Control vs IL-22 stimulation |
| GSM1055657 |
Control vs Doxycycline addition (Hes1 overexpression) |
| GSM1055658 |
Control vs IL22 stimulation+Doxycycline addition (Hes1 overexpression) |
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| Relations |
| BioProject |
PRJNA184194 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43012_RAW.tar |
8.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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