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| Status |
Public on May 01, 2013 |
| Title |
RNASET2-silenced and control OVCAR3 cell clones injected into nude mice. |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the molecular pathways underlying RNASET2-mediated tumor suppression in vivo, we investigated the gene expression profile of tumor samples obtained from control and RNASET2-silenced OVCAR3 xenografts, respectively. In order to evaluate the contribution of human cancer cells and the murine host stroma to RNASET2-mediated tumorigenesis responses, total RNA extracted from tumor xenografts derived from six independent OVCAR3 cell clones (three parental and three RNASET2-silenced clones) was fluorescently labeled and hybridized to human Agilent whole-genome microarrays.
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| Overall design |
Control and RNASET2-silenced OVCAR3 were injected subcutaneously into nude mice. After 39 days mice were sacrified, tumors were extracted and total RNA purified.
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| Contributor(s) |
Monti L, Sacco MG, Collotta A, Fabbri M, Gribaldo L, Acquati F, Taramelli R |
| Citation(s) |
23630276 |
| Submission date |
Dec 19, 2012 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Marco Fabbri |
| E-mail(s) |
fabbri.marco@gmail.com
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| Organization name |
Università dell'Insubria
|
| Street address |
via dunant 5
|
| City |
Varese (VA) |
| ZIP/Postal code |
21100 |
| Country |
Italy |
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| Platforms (2) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA184132 |
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