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Series GSE4307 Query DataSets for GSE4307
Status Public on Apr 26, 2006
Title Expression data from single cells from ICMs of mouse blastocysts at E3.5
Organism Mus musculus
Experiment type Expression profiling by array
Summary The inner cell mass (ICM) of the early blastocyst at E3.5, a source of ES cell derivation, is a morphologically homogeneous population of undifferentiated pluripotent cells that give rise to all embryonic lineages. The immediate application of the newly developed V1V3 method to single cells in this stage of mouse embryos revealed the presence of two populations of cells, one with primitive endoderm expression and the other with pluripotent epiblast-like gene expression. The genes expressed differentially between these two populations were well preserved in morphologically differentiated primitive endoderm and epiblast in the embryos one day later (E4.5), demonstrating that the method successfully detects subtle but essential differences in gene expression at the single-cell level among seemingly homogeneous cell populations. This study provides a strategy to analyze biophysical events in medicine as well as in neural, stem cell, and developmental biology, where small numbers of distinctive or diseased cells play critical roles.
Keywords: Single cell analysis
 
Overall design We isolated blastocysts at E3.5 and dissociated the ICM into single cells by trypsin-EDTA treatment. To prepare cDNA samples, we then randomly picked a total of 55 single cells. cDNAs were synthesized and amplified by the V1V3 method, and screened by gene-specific PCR using Oct4 and Cdx2 to remove trophectoderm cells, and 50 cells were identified as Oct4-positive and Cdx2-negative, ICM cells.
 
Contributor(s) Kurimoto K, Yabuta Y, Ohinata Y, Ono Y, Uno KD, Yamada RG, Ueda HR, Saitou M
Citation(s) 16547197
Submission date Feb 27, 2006
Last update date Feb 11, 2019
Contact name Kazuki Kurimoto
E-mail(s) kurimoto@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University
Department Graduate school of medicine
Lab Department of anatomy and cell biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (20)
GSM98548 single cell from ICM E3.5, biological rep1
GSM98549 single cell from ICM E3.5, biological rep2
GSM98550 single cell from ICM E3.5, biological rep3
This SubSeries is part of SuperSeries:
GSE4309 An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
Relations
BioProject PRJNA104667

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4307_RAW.tar 113.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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