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Series GSE4308 Query DataSets for GSE4308
Status Public on Apr 26, 2006
Title Expression data for validation of single cell cDNA amplification method (V1V3 method)
Organism Mus musculus
Experiment type Expression profiling by array
Summary A systems-level understanding of a small but essential population of cells in development or adulthood (e.g., somatic stem cells) requires accurate quantitative monitoring of genome-wide gene expression, ideally from single cells. We report here a strategy to globally amplify mRNAs from single cells for highly quantitative high-density oligonucleotide microarray analysis that combines a small number of directional PCR cycles with subsequent linear amplification. Using this strategy, both the representation of gene expression profiles and reproducibility between individual experiments are unambiguously improved from the original method, along with high coverage and accuracy.
Keywords: Method validation
Overall design To verify the ability of the developed method, we prepared total RNA from ES cells and diluted it to the single-cell level (10 pg). Eight array data from the cDNA samples amplified independently from the 10 pg RNA (amplified samples) were compared to each other, and to eight array data from the undiluted 5 ug RNA (nonamplified controls). Since all the materials were the same, all of the observed variations were attributable to the methodogoly.
Contributor(s) Kurimoto K, Yabuta Y, Ohinata Y, Ono Y, Uno KD, Yamada RG, Ueda HR, Saitou M
Citation(s) 16547197
Submission date Feb 27, 2006
Last update date Feb 11, 2019
Contact name Kazuki Kurimoto
Organization name Kyoto University
Department Graduate school of medicine
Lab Department of anatomy and cell biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM98568 ES 10pg amplified, technical rep1
GSM98569 ES 10pg amplified, technical rep2
GSM98570 ES 10pg amplified, technical rep3
This SubSeries is part of SuperSeries:
GSE4309 An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
BioProject PRJNA104647

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Supplementary file Size Download File type/resource
GSE4308_RAW.tar 93.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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