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Series GSE43154 Query DataSets for GSE43154
Status Public on Dec 27, 2012
Title Comparison of RelB-/- and RelB+/- thymi
Organism Mus musculus
Experiment type Expression profiling by array
Summary Thymic medullary epithelial cell (mTEC) expression of the autoimmune regulator AIRE, and of tissue-specific antigens, is controlled by members of the non-canonical NF-kB signalling pathway, including RelB and NF-kB2. Of the genes in this pathway, RelB-/- mice develop a particularly severe multi-organ autoimmune syndrome, resembling Foxp3-deficiency. RelB-/- mice have medullary atrophy and few mTECs but the mechanism is unknown. We show that RelB is required for expression of medullary chemokines and mTEC AIRE, selection of a diverse peripheral T cell repertoire, and for peripheral Foxp3+ Treg function. Vβ families of T cells infiltrating diseased peripheral organs and thymic Treg were similarly skewed. Surprisingly, medullary atrophy results from intra-thymic granulocyte infiltration, consequent upon the Th2-mediated autoimmune disease. Dominant tolerance corrects thymic inflammatory disease and loss of thymic function. We demonstrate a reversible RelB-dependent inflammatory mechanism for loss of central tolerance associated with medullary atrophy.
 
Overall design Thymi from 4 RelB+/- mice and 3 RelB-/- mice were profiled by microarays
 
Contributor(s) O'Sullivan BJ
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Submission date Dec 26, 2012
Last update date Jun 14, 2018
Contact name Brendan John O'Sullivan
Organization name The University of Queensland Diamantina Institute Translational Research Institute
Street address Level 6, 37 Kent Street Woolloongabba
City Brisbane
State/province Queensland
ZIP/Postal code 4102
Country Australia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (7)
GSM1057544 Thymus wt 1
GSM1057545 Thymus ko 1
GSM1057546 Thymus wt 2
Relations
BioProject PRJNA184731

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43154_RAW.tar 3.1 Mb (http)(custom) TAR
GSE43154_non-normalized.txt.gz 1.2 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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