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Series GSE43221 Query DataSets for GSE43221
Status Public on Dec 31, 2012
Title Transcript levels in CCE WT and RARgamma knockout murine embryonic stem cells treated with either all-trans retinoic acid (8 and 24 hr) or with vehicle control
Organism Mus musculus
Experiment type Expression profiling by array
Summary Retinoic acid receptors (RARs) α, β, and γ heterodimerize with Retinoid X receptors (RXR) α, β, and γ and bind the cis-acting response elements known as RAREs to execute the biological functions of retinoic acid during mammalian development. RARγ mediates the anti-proliferative and apoptotic effects of retinoids in certain tissues and cancer cells, such as melanoma and neuroblastoma cells. Furthermore, ablation of RARγ enhanced the tumor incidence of Ras transformed keratinocytes and was associated with resistance to retinoid mediated growth arrest and apoptosis.
We used microarray analysis to identify genes, which upon 8 or 24 hr of treatment with all-trans retinoic acid display differential expression in RARγ knockout (RARγKO) murine embryonic stem cells relative to CCE WT cells. We demonstrate that following RA treatment the majority of inducible transcripts are present at lower levels in RARγKO ES cells compared to WT ES cells.
 
Overall design Murine embryonic stem cells (WT and RARγKO) were treated with either all-trans retinoic acid (up to 24 hr) or with vehicle control (EtOH).
 
Contributor(s) Laursen KB, Gudas LJ
Citation(s) 23264745
Submission date Dec 31, 2012
Last update date Jun 14, 2018
Contact name Kristian Bruun Laursen
E-mail(s) krl2004@med.cornell.edu
Organization name Weill Cornell Medical College
Department Pharmacology
Lab Lorraine Gudas
Street address 1300 York Avenue
City New York
ZIP/Postal code NY 10065
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM1058922 CCE_WT_Ctrl_1
GSM1058923 CCE_WT_Ctrl_2
GSM1058924 CCE_WT_Ctrl_3
Relations
BioProject PRJNA185004

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43221_RAW.tar 3.1 Mb (http)(custom) TAR
GSE43221_non-normalized_data.txt.gz 12.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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