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| Status |
Public on Aug 27, 2013 |
| Title |
Effects of supernatants of USP2 knockdown HL-60 cells and mock transfected HL60 cells on differentiated 3T3-L1 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Ubiquitin specifc protease (USP) 2 modulates various cellular responces including carcinogenesis, spermatogenesis, and glucose metabolism. So far, we established USP2 knockdown HL-60 cells to explore roles of USP2 in macrophages. In this experiment, we investigated whether USP2 knockdown HL-60 cells more prominently modulate function of adipocytes compared with control cells.
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| Overall design |
USP2 knock down(KD) HL-60 cells and mock vector-transfected mock control cells were differentiated into macrophage-like cells in the presence of 30nM of phorbol 12-myristate 13-acetate for 5 days. Subseqently, supernatants of the USP2 KD cells and control cells were added to differentiated 3T3-L1 cells for 3 and 6 hrs. Total RNA was isolated from the supernatant-treated 3T3-L1 cells, and subjected to microarray analysis. Duplicate samples were prepared for each cell and time point.
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| Contributor(s) |
Kitamura H, Shimamoto Y, Naoe Y |
| Citation(s) |
24005904 |
| Submission date |
Jan 07, 2013 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Hiroshi Kitamura |
| E-mail(s) |
ktmr@rakuno.ac.jp
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| Phone |
81-11-388-4781
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| Organization name |
Rakuno Gakuen University
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| Department |
School of Veterinary Medicine
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| Lab |
Laboratory for Veterinary Physiology
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| Street address |
582 Midorimachi, Bunkyodai
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| City |
Ebetsu |
| ZIP/Postal code |
069-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA185411 |
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