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| Status |
Public on Apr 14, 2015 |
| Title |
Inhibitory effects of galacturonic acid on Saccharomyces cerevisiae: involvement of hexose transporters |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Aim: Analyse inhibitory effects of galacturonic acid, an important constituent of plant biomass hydrolysates, on growing and starving cultures of Saccharomyces cerevisiae CEN.PK113-7D. Method & Results: Biomass yields in aerobic and anaerobic glucose-limited chemostat cultures (pH 3.5) were reduced by 25 and 10%, respectively, upon addition of 10 g∙l-1 galacturonic acid. Genes previously reported to show a transcriptional response to other organic acids were overrepresented in a set of galacturonic-acid responsive genes identified by microarray analysis. These results suggested that galacturonic acid causes weak-acid uncoupling of the yeast plasma membrane pH gradient. Consistent with this hypothesis, galacturonate-accelerated loss of viability in starving cell suspensions was strongly pH dependent. Loss of viability was much slower in a strain in which all HXT (hexose transporter) genes were deleted. Moreover, deletion of HXT genes alleviated growth inhibition on ethanol observed at galacturonic acid concentrations of 10 g∙l-1 and above. Conclusions: At low pH, galacturonic acid negatively affects the physiology of S. cerevisiae. Reduced sensitivity of hexose-transporter mutants indicated that one or more HXT transporters are involved in transport of galacturonic acid. Significance and Impact: This study shows that galacturonic acid toxicity should be taken into account in process development for yeast-based fermentative conversion of pectin-rich feedstocks such as sugar beet pulp and citrus peel. Involvement of hexose transporters in galacturonic acid toxicity provides leads for improving tolerance.
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| Overall design |
To investigate the impact of galacturonic acid on S. cerevisiae, a DNA microarray-based transcriptome analysis was performed on aerobic, glucose-limited chemostat cultures grown in the presence and absence of 10 g∙l-1 galacturonic acid at pH3.5.
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| Contributor(s) |
Huijses E, van Maris AJ, Pronk JT, Daran J |
| Citation missing |
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| Submission date |
Jan 14, 2013 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Jean-Marc Daran |
| E-mail(s) |
j.g.daran@tudelft.nl
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| Phone |
+31 15 278 2412
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| Organization name |
Delft University of Technology
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| Department |
Department of Biotechnology
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| Lab |
Kluyver centre for genomics of industrial organisms
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| Street address |
Julianalaan 67
|
| City |
Delft |
| ZIP/Postal code |
2628BC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
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| Samples (7)
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| GSM1063367 |
CEN.PK113-7D Aerobic glucose-limited chemostat pH3.5 (EH5) |
| GSM1063368 |
CEN.PK113-7D Aerobic glucose-limited chemostat pH3.5 (EH6) |
| GSM1063369 |
CEN.PK113-7D Aerobic glucose-limited chemostat pH3.5 (EH7) |
| GSM1063370 |
CEN.PK113-7D Aerobic glucose-limited chemostat 10g/l GalU pH3.5 (EH4) |
| GSM1063371 |
CEN.PK113-7D Aerobic glucose-limited chemostat 10g/l GalU pH3.5 (EH3) |
| GSM1063372 |
CEN.PK113-7D Aerobic glucose-limited chemostat pH3.5 0.23g/l galactose (MA185R2) |
| GSM1063373 |
CEN.PK113-7D Aerobic glucose-limited chemostat pH3.5 0.23g/l galactose (MA186R2) |
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| Relations |
| BioProject |
PRJNA186523 |
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