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| Status |
Public on Jan 16, 2013 |
| Title |
Comparison of gene expression signature between nascent Lhx6-GFP+ cells from mouse embryonic brain and differentiated Lhx6-GFP+ cells from ES cells (as well as comparing those of Lhx6-GFP+ and Lhx6-GFP- cells from ES cells) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
There was a remarkable similarity in the molecular properties of the MGE-GFP+ and ES-GFP+ cells. In particular, genes that are important for medial ganglionic eminence (MGE) and cortical interneurons development are both high in expression in both MGE-Lhx6-GFP+ and ES-Lhx6-GFP+ cells (compared to ES-Lhx6-GFP- cells).
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| Overall design |
To investigate how closely ES cells-derived Lhx6-GFP+ cells resembled authentic Lhx6+ MGE cells, and to define the molecular properties of the Lhx6-GFP+ and Lhx6-GFP- cells from differentiated ES cells, we compared their gene expression profiles. We used FACS to purify GFP+ cells from the E12.5 MGE of Lhx6-GFP transgenic mice. ES-Lhx6-GFP+ cells and ES-Lhx6-GFP- cells (both from D12 EB aggregates) were also isolated by fluorescent activated cell sorting (FACS) and all of the RNA samples were subjected to RNA expression microarray analyses.
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| Contributor(s) |
Rubenstein J, Chen J |
| Citation(s) |
23658702 |
| Submission date |
Jan 15, 2013 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Joshua Pollack |
| E-mail(s) |
joshua.pollack@pionyrtx.com
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| Organization name |
Pionyr Immunotherapeutics
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| Street address |
2 Tower Pl. Ste 800
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA186616 |
