|
| Status |
Public on Mar 02, 2013 |
| Title |
H3K4me3 Interactions with TAF3 Regulate Preinitiation Complex Assembly and Selective Gene Activation [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Histone modifications regulate chromatin-dependent processes, yet the mechanisms by which they contribute to specific outcomes remain unclear. H3K4me3 is a prominent histone mark that is associated with active genes and promotes transcription through interactions with effector proteins that include initiation factor TFIID. We demonstrate that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that (i) H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex (PIC) formation; (ii) H3K4me3, through TAF3 interactions, can act either independently or cooperatively with the TATA box to direct PIC formation and transcription; and (iii) H3K4me3-TAF3/TFIID interactions regulate gene-selective functions of p53 in response to genotoxic stress. Our findings indicate a mechanism by which H3K4me3 directs PIC assembly for the rapid induction of specific p53 target genes
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| |
|
| Overall design |
Examination of TAF3, RNAPII, and H3K4me3 distribution in HCT116 cells.
|
| |
|
| Contributor(s) |
Lauberth SM, Nakayama T, Wu X, Ferris A, Hughes SH, Roeder RG |
| Citation(s) |
23452851 |
| Submission date |
Jan 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiaolin Wu |
| E-mail(s) |
forestwu@mail.nih.gov
|
| Phone |
301-846-7677
|
| Organization name |
Frederick National Laboratory for Cancer Research/SAIC-Frederick Inc
|
| Department |
Cancer Research Technology Program
|
| Lab |
Genomics Laboratory
|
| Street address |
8560 Progress Drive, C3001
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21701 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (4)
|
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| This SubSeries is part of SuperSeries: |
| GSE43542 |
H3K4me3 Interactions with TAF3 Regulate Preinitiation Complex Assembly and Selective Gene Activation |
|
| Relations |
| BioProject |
PRJNA186696 |
| SRA |
SRP017967 |
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