|
| Status |
Public on Dec 09, 2013 |
| Title |
Evaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq technique was applied to investigate the effects of two semen collection methods (Pelleted vs Liquefied) and two sperm purification methods (SCLB vs PS) to the integrity of isolated RNAs at different perspectives.
|
| |
|
| Overall design |
The same set of semen samples were applied to investigate the qualitative and quantitative effect of semen collection methods and sperm cell purification methods on sperm transcript profiling.
|
| |
|
| Contributor(s) |
Mao S, Goodrich RJ, Hauser R, Krawetz SA |
| Citation(s) |
23869956 |
| Submission date |
Jan 17, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen Krawetz |
| E-mail(s) |
steve@compbio.med.wayne.edu
|
| Phone |
313-577-6770
|
| Organization name |
Wayne State University School of Medicine
|
| Department |
Center for Molecular Medicine and Genetics
|
| Lab |
C.S. Mott Center for Human Growth and Development
|
| Street address |
275 E. Hancock
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA186783 |
| SRA |
SRP018020 |
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