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Series GSE43658 Query DataSets for GSE43658
Status Public on Apr 01, 2013
Title Transcriptional co-factor TBLR1 controls lipid mobilization in white adipose tissue
Organism Mus musculus
Experiment type Expression profiling by array
Summary Lipid mobilization (lipolysis) in white adipose tissue (WAT) critically controls lipid turnover and adiposity in humans. While the acute regulation of lipolysis has been studied in detail, the transcriptional determinants of WAT lipolytic activity remain still largely unexplored. Here we show that the genetic inactivation of transcriptional co-factor transducin beta-like-related (TBLR) 1 blunts the lipolytic response of white adipocytes through the impairment of cAMP-dependent signal transduction. Indeed, mice lacking TBLR1 in adipocytes are defective in fasting-induced lipid mobilization and when placed on a high fat diet show aggravated adiposity, glucose intolerance and insulin resistance. TBLR1 levels are found to increase under lipolytic conditions in WAT of both human patients and mice, correlating with serum free fatty acids (FFA). As a critical regulator of WAT cAMP signaling and lipid mobilization, proper activity of TBLR1 in adipocytes may thus represent a critical molecular checkpoint for the prevention of metabolic dysfunction in subjects with obesity-related disorders.
We used microarrays to identify global gene expression in 3T3-L1 adipocytes lacking TBLR1 and compared gene expression to control shRNA treated cells in both basal and isoproterenol stimulated states.
Overall design We analyzed 12 RNA samples extracted from 3T3-L1 adipocytes that were treated with either control or TBLR1 specific shRNAs and with or without 10 µM isoproterenol for 3 hrs. Three replicates of each condition.
Contributor(s) Rohm M, Sommerfeld A, Strzoda D, Jones A, Sijmonsma T, Rudofsky G, Wolfrum C, Sticht C, Gretz N, Zeyda M, Leitner L, Nawroth P, Stulnig T, Berriel Diaz M, Vegiopoulos A, Herzig S
Citation(s) 23499424
Submission date Jan 22, 2013
Last update date Jun 16, 2014
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
Platforms (1)
GPL14661 [Mouse430_2_Mm_EntrezG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 13]
Samples (12)
GSM1067681 Negative control shRNA transduced cells, not treated with isoproterenol, replicate 1
GSM1067682 Negative control shRNA transduced cells, not treated with isoproterenol, replicate 2
GSM1067683 Negative control shRNA transduced cells, not treated with isoproterenol, replicate 3
BioProject PRJNA186944

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Supplementary file Size Download File type/resource
GSE43658_RAW.tar 42.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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