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Series GSE43691 Query DataSets for GSE43691
Status Public on May 01, 2013
Title Hepatic molecular alterations more than muscle's differentiate obese hyperphagic mice from those prolonged fed with the high-fat diet
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although mitochondrial dysfunctions are implicated in the pathogenesis of obesity, the molecular mechanisms underlying obesity-related metabolic abnormalities are still not well established. To acquire a comprehensive picture of mitochondrial molecular changes within metabolically active tissues, we focused on hepatic and muscle whole cellular transcriptome and mitochondrial proteome alterations in 16 and 48 weeks old high fat diet (HFD)-feed wild type C57BL/6J and hyperphagic, genetically modified mice with leptin dysfunction (ob/ob and db/db).
On transcriptome level, the most discriminative hepatic alterations distinguished between genetically modified and wild type mice, and between overnight fasted and non-fasted mice, while the muscle transcriptional alterations related mainly to the fasting state. The fractions of uniquely different proteins were consistently higher in hyperphagic than in HFD-fed mice and in fasted than non-fasted mice . The liver samples revealed overall higher number of differentially expressed RNAs and proteins than muscle samples. Differentially expressed genes and proteins in the liver, but not in the muscle, could be assigned to several Gene Ontology terms, including oxidation-reduction and several metabolic processes. Thus, altered expression of genes and proteins accompanied the state of obesity and was quantitatively different in the liver and muscle.
Our parallel microarray- and quantitative mitochondrial mass spectrometry-based study performed on hepatic and muscle samples identified a higher number of differentially expressed proteins than any other studies investigating obesity-related proteomes. However, even with our integrated transcriptomic and proteomic approach still many details and dynamics of a chain of metabolic events leading to obesity-related mitochondrial dysfunctions remain unresolved .
 
Overall design 48 samples each of liver and muscle (total samples: 96). Samples splitted equally according to 3 criterions: age (24 young/24 old), fasting status (24 fasted/24 non fasted), and strain+diet (12 each: B6.V-Lep ob/J+D12450B, B6.BKS(D)-Lep rdb/J+D12450B, C57BL/6J+D12450B, C57BL/6J+D12492). Overall 3 replicates for each strain+diet/age/fasting_status combination. Low fat diet = D12450B; high fat diet = D12492.
 
Contributor(s) Nesteruk M, Hennig EE, Mikula M, Karczmarski J, Dzwonek A, Goryca K, Rubel T, Paziewska A, Woszczynski M, Ledwon J, Dabrowska M, Walewska-Zielecka B, Pysniak K, Dadlez M, Ostrowski J
Citation(s) 24178926
Submission date Jan 23, 2013
Last update date Sep 18, 2020
Contact name Krzysztof Goryca
Organization name Centrum Onkologii Instytut im. Marii Skłodowskiej Curie
Department Department of Genetics
Street address Roentgena 5
City Warszawa
ZIP/Postal code 02-781
Country Poland
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (96)
GSM1068282 ob_16 weeks_non-fasted_rep_1 [liver]
GSM1068283 ob_16 weeks_non-fasted_rep_2 [liver]
GSM1068284 ob_16 weeks_non-fasted_rep_3 [liver]
Relations
BioProject PRJNA187067

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43691_RAW.tar 3.1 Mb (http)(custom) TAR
GSE43691_non-normalized_data_Liver.txt.gz 4.6 Mb (ftp)(http) TXT
GSE43691_non-normalized_data_Muscle.txt.gz 4.6 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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