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Series GSE43692 Query DataSets for GSE43692
Status Public on Jan 24, 2013
Title Genome-wide microarray analysis of normal human fibroblasts in response to kaemferol, daidzein, kaemferol/genistein, and daidzein/genistein
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Establishment of a transcriptomic profile of human cells treated with kaemferol, daidzein, kaemferol/genistein, or daidzein/genistein with particular emphasis on signature of genes coding for enzymes involved in glycosaminoglycan synthesis stands for the present study. The hypothesis tested was that kaemferol, daidzein, kaemferol/genistein, and daidzein/genistein influence expression of some genes, among which are those coding for enzymes required for the synthesis of different GAGs being pathologically accumulated in mucopolysaccharidoses. Results provide important information concerning the extent of action of kaemferol, daidzein, kaemferol/genistein, and daidzein/genistein at the molecular level in terms of modulation of gene expression.
 
Overall design Total RNA was obtained from human dermal fibroblasts (HDFa) subjected to 24 or 48 hours in vitro exposure to 30, 60 or 100 µM kaemferol; 60 or 100 µM daidzein; 30 µM + 30 µM kaemferol/genistein; 30 µM + 30 µM daidzein/genistein; or 0.05%DMSO. Three replicates each.
 
Contributor(s) Gabig-Cimińska M
Citation(s) 25797591, 26209177
Submission date Jan 23, 2013
Last update date Oct 09, 2019
Contact name Magdalena Gabig-Cimińska
E-mail(s) m.gabig@biol.ug.edu.pl
Organization name University of Gdańsk
Department of Molecular Biology
Street address Wita Stwosza 59
City Gdańsk
ZIP/Postal code 80-308
Country Poland
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (48)
GSM1068330 Fibroblast_30 µM kaemferol_24h_rep1_rep1
GSM1068331 Fibroblast_60 µM kaemferol_24h_rep1
GSM1068332 Fibroblast_100 µM kaemferol _24h_rep1
Relations
BioProject PRJNA187064

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43692_RAW.tar 26.2 Mb (http)(custom) TAR
GSE43692_non-normalized_rep1.txt.gz 2.6 Mb (ftp)(http) TXT
GSE43692_non-normalized_rep2.txt.gz 2.6 Mb (ftp)(http) TXT
GSE43692_non-normalized_rep3.txt.gz 2.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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