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| Status |
Public on Mar 01, 2013 |
| Title |
Gene expression profiles of MCF7 breast cancer cells following estrogen and anti-estrogen treatment. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have previously demonstrated that endoxifen is the most important tamoxifen metabolite responsible for eliciting the anti-estrogenic effects of this drug in breast cancer cells expressing estrogen receptor-alpha. The goals of this study were to compare the gene expression profiles elicited by endoxifen to that of other anti-estrogens in MCF7 cells. We also examined the gene expression profiles elicited by various endoxifen concentrations in the presence of tamoxifen and its other primary metabolites in order to better understand the molecular contributions of endoxifen to the effects of tamoxifen.
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| Overall design |
Total RNA was isolated from parental MCF7 cells following 24 hour treatments with various individual or combined ligands. All studies were conducted in replicates of 3.
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| Contributor(s) |
Hawse JR |
| Citation(s) |
23382923 |
| Submission date |
Jan 23, 2013 |
| Last update date |
Aug 16, 2018 |
| Contact name |
John Hawse |
| E-mail(s) |
hawse.john@mayo.edu
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| Phone |
507-284-4268
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| Organization name |
Mayo Clinic
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| Department |
Biochemistry and Molecular Biology
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| Street address |
200 1st St. SW
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| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (38)
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| GSM1068630 |
MCF7, 100 nM 4HT, 24 h, rep 1 |
| GSM1068631 |
MCF7, 100 nM 4HT, 24 h, rep 2 |
| GSM1068632 |
MCF7, 100 nM 4HT, 24 h, rep 3 |
| GSM1068633 |
MCF7, 100 nM endoxifen, 24 h, rep 2 |
| GSM1068634 |
MCF7, 100 nM endoxifen, 24 h, rep 3 |
| GSM1068635 |
MCF7, 100 nM ICI, 24 h, rep 1 |
| GSM1068636 |
MCF7, 100 nM ICI, 24 h, rep 2 |
| GSM1068637 |
MCF7, 100 nM ICI, 24 h, rep 3 |
| GSM1068638 |
MCF7, vehicle, 24 h, rep 4 |
| GSM1068639 |
MCF7, vehicle, 24 h, rep 5 |
| GSM1068640 |
MCF7, vehicle, 24 h, rep 6 |
| GSM1068641 |
MCF7, 10 nM estradiol, 24 h, rep 1 |
| GSM1068642 |
MCF7, 10 nM estradiol, 24 h, rep 2 |
| GSM1068643 |
MCF7, 10 nM estradiol, 24 h, rep 3 |
| GSM1068644 |
MCF7, 10 nM estradiol + 100 nM 4HT, 24 h, rep 1 |
| GSM1068645 |
MCF7, 10 nM estradiol + 100 nM 4HT, 24 h, rep 2 |
| GSM1068646 |
MCF7, 10 nM estradiol + 100 nM 4HT, 24 h, rep 3 |
| GSM1068647 |
MCF7, 10 nM estradiol + 100 nM endoxifen, 24 h, rep 1 |
| GSM1068648 |
MCF7, 10 nM estradiol + 100 nM endoxifen, 24 h, rep 2 |
| GSM1068649 |
MCF7, 10 nM estradiol + 100 nM endoxifen, 24 h, rep 3 |
| GSM1068650 |
MCF7, 10 nM estradiol + 100 nM ICI, 24 h, rep 1 |
| GSM1068651 |
MCF7, 10 nM estradiol + 100 nM ICI, 24 h, rep 2 |
| GSM1068652 |
MCF7, 10 nM estradiol + 100 nM ICI, 24 h, rep 3 |
| GSM1068653 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen, 24 h, rep 1 |
| GSM1068654 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen, 24 h, rep 2 |
| GSM1068655 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen, 24 h, rep 3 |
| GSM1068656 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 1000 nM endoxifen, 24 h, rep 1 |
| GSM1068657 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 1000 nM endoxifen, 24 h, rep 2 |
| GSM1068658 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 1000 nM endoxifen, 24 h, rep 3 |
| GSM1068659 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 100 nM endoxifen, 24 h, rep 1 |
| GSM1068660 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 100 nM endoxifen, 24 h, rep 2 |
| GSM1068661 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 100 nM endoxifen, 24 h, rep 3 |
| GSM1068662 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 20 nM endoxifen, 24 h, rep 1 |
| GSM1068663 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 20 nM endoxifen, 24 h, rep 2 |
| GSM1068664 |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 20 nM endoxifen, 24 h, rep 3 |
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| Relations |
| BioProject |
PRJNA187086 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43702_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR |
| GSE43702_non-normalized.txt.gz |
10.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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