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Series GSE43856

Query DataSets for GSE43856

Status

Public on Jan 30, 2013

Title

Changes in the transcriptome of circulating neutrophils and skeletal muscle in response to endurance exercise

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

Analysis of the changes in the transcriptome of circulating neutrophils and skeletal muscle from standardized resting conditions (baseline; pre-EXTRI) to 3, 48 and 96 hours after an experimental exercise trial (EXTRI; 1 hour of cycling followed by 1 hours of running) in 8 healthy, endurance-trained, male subjects. It was hypothesized that the time-course dependent transcriptomic changes would reflect the molecular and signalling mechanisms by which neutrophils regulate and counter-regulate inflammation, and by which skeletal muscle responds, regenerates, and phenotypically adapts to intense, prolonged exercise involving muscle damage. Results provide an important insight in the signalling pathways underlying the transcriptional activation and priming of circulatory neutrophils in response to physiological stress, in particular muscle-derived damage-associated molecular patterns. Furthermore, the study provides novel data on the skeletal muscle transcriptome beyond 48 hours after strenuous endurance exercise, and indicates important muscular remodelling processes at 96 hours post-EXTRI.

Overall design

Blood and muscle samples were taken under standardized conditions at baseline (pre-EXTRI), and 3, 48 and 96 hours post-EXTRI. Total mRNA was extracted from isolated neutrophils, and from skeletal muscle tissue.

Contributor(s)

Neubauer O, Sabapathy S, Lazarus R, Jowett JB, Asthon K, Desbrow B, Peake JM, Cameron-Smith D, Haseler LJ, Wagner K, Bulmer AC

Citation(s)

23580600

Submission date

Jan 29, 2013

Last update date

Aug 13, 2018

Contact name

Oliver Neubauer

E-mail(s)

oliver.neubauer@univie.ac.at