|
Status |
Public on Jun 13, 2014 |
Title |
Mtg16 regulates E protein activity and lineage specification in dendritic cell development (ChIP-seq) |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
E protein transcription factors specify major immune cell lineages including lymphocytes and interferon-producing plasmacytoid dendritic cells (pDCs). Corepressors of the ETO family can bind to and block transactivation by E proteins, but the physiological role of these interactions remained unclear. We report that ETO protein Mtg16 binds chromatin primarily through the pDC-specific E protein E2-2 in human pDCs. Mtg16-deficient mice showed impaired pDC development and functionality, whereas the specification of the classical dendritic cells (cDCs) was enhanced. The deletion of Mtg16 caused aberrant expression of E protein antagonist Id2 in pDCs. Thus, Mtg16 acts as a cofactor of E2-2 to promote pDC differentiation and restrict cDC development, revealing an unexpected positive role of ETO proteins in E protein activity.
|
|
|
Overall design |
Analysis of E2-2 and Mtg16 immunoprecipitated chromatin from CAL-1 cell line.
|
|
|
Contributor(s) |
Ghosh HS, Reizis B |
Citation(s) |
24980046 |
Submission date |
Jan 29, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Hiyaa Singhee Ghosh |
E-mail(s) |
hg2238@columbia.edu
|
Organization name |
Columbia University Medical Center
|
Street address |
701 W 168th Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
Samples (3) |
|
This SubSeries is part of SuperSeries: |
GSE43963 |
Mtg16 regulates E protein activity and lineage specification in dendritic cell development |
|
Relations |
BioProject |
PRJNA188233 |
SRA |
SRP018349 |