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| Status |
Public on Jul 08, 2013 |
| Title |
CD8 T cells induce perforin-dependent pathology in Leishmania braziliensis infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The mechanisms that mediate immunopathologic responses in infectious diseases are often less well understood than how the pathogens are controlled. Here, we have investigated what causes increased pathology following infection with the protozoan parasite, Leishmania braziliensis. We focused on CD8 T cells since their presence in leishmanial lesions has been correlated with increased disease. By adoptively transferring CD8 T cells to L. braziliensis infected RAG mice, we found that unregulated CD8 T cells promote severe pathology at the infection site, as well as the development of metastatic lesions in other skin sites. In mice with severe pathology, we visualized CD8 T cells degranulating and lysing L. braziliensis infected cells, and in parallel studies with L. braziliensis patients we confirmed that CD8 T cells within lesions exhibit a cytolytic phenotype. We found that perforin deficient CD8 T cells failed to induce disease, indicating that the increased disease induced by CD8 T cells was due perforin-dependent cytotoxicity. In contrast, although we found that CD8 T cells made both IFN-γ and IL-17, neither of these cytokines is required for the development of pathology. Thus, we show for the first time that immunopathology in leishmaniasis can be mediated by cytolytic CD8 T cells.
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| Overall design |
Twelve skin biopsy samples were analyzed, including 2 normal skin biopsies obtained from patients in N. America, and 10 skin biosies obtained from Leishmania brazilensis infected patients presenting at the Corte de Pedra Health Post in Corte de Pedra, Bahia, Brazil
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| Contributor(s) |
Beiting D, Scott P, Novais F, Carvalho E, Carvalho L |
| Citation(s) |
23874205, 24403561, 25393535 |
| Submission date |
Jan 29, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
daniel beiting |
| E-mail(s) |
beiting@vet.upenn.edu
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| Organization name |
University of Pennsylvania
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| Department |
Pathobiology
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| Lab |
Beiting
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| Street address |
380 S. University Ave
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA187994 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43880_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE43880_non-normalized.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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