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Series GSE43924 Query DataSets for GSE43924
Status Public on Jan 21, 2015
Title Global gene expression analysis of human iPSC-derived Neural Stem Cells (NSC) from LCA patients and unaffected persons
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Our purpose was to investigate genes and molecular mechanisms involved in patients with Leber congenital amaurosis (LCA). Fibroblasts from two unrelated clinically-identified patients (Coriell) were reprogrammed to pluripotency by retroviral transduction. These human induced Pluripotent Stem Cells (hiPSCs) were differentiated into neural stem cells (NSC) that mimicked the neural tube stage and retinal pigmented epithelial (RPE) cells that could be targeted by the disease. A genome wide transcriptome analysis was performed with Affymetrix Exon Array GeneChip®, comparing LCA-hiPSCs derivatives to controls. The aim was to identify differentially expressed genes which may be associated with early developmental defect before the establishment of mature retinal circuitry.
 
Overall design We analyzed iPSC-derived neural stem cells from LCA patient's fibroblast (n=2) and iPSC-derived neural stem cells from healthy people fibroblast (n=2). A total of 21 samples were analyzed : 9 NSC derived from iPSC LCA and 12 NSC derived from wild-type iPSC.
 
Contributor(s) Lustremant C, de la Grange P, Monville C
Citation(s) 23663011
Submission date Jan 30, 2013
Last update date Feb 18, 2019
Contact name Pierre de la Grange
E-mail(s) pierre.delagrange@genosplice.com
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platforms (1)
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (21)
GSM1074467 hiPSC GM03852cl1-derived Neural Stem Cells at passage 7, biological rep1
GSM1074468 hiPSC GM03852cl1-derived Neural Stem Cells at passage 7, biological rep2
GSM1074469 hiPSC GM03852cl1-derived Neural Stem Cells at passage 7, biological rep3
This SubSeries is part of SuperSeries:
GSE43946 Human induced pluripotent stem cells reveal early developmental molecular correlates with a probable Leber congenital amaurosis type I
Relations
BioProject PRJNA188200

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Supplementary file Size Download File type/resource
GSE43924_RAW.tar 521.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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