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Status |
Public on Jan 21, 2015 |
Title |
Global gene expression analysis of human iPSC-derived Neural Stem Cells (NSC) from LCA patients and unaffected persons |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Our purpose was to investigate genes and molecular mechanisms involved in patients with Leber congenital amaurosis (LCA). Fibroblasts from two unrelated clinically-identified patients (Coriell) were reprogrammed to pluripotency by retroviral transduction. These human induced Pluripotent Stem Cells (hiPSCs) were differentiated into neural stem cells (NSC) that mimicked the neural tube stage and retinal pigmented epithelial (RPE) cells that could be targeted by the disease. A genome wide transcriptome analysis was performed with Affymetrix Exon Array GeneChip®, comparing LCA-hiPSCs derivatives to controls. The aim was to identify differentially expressed genes which may be associated with early developmental defect before the establishment of mature retinal circuitry.
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Overall design |
We analyzed iPSC-derived neural stem cells from LCA patient's fibroblast (n=2) and iPSC-derived neural stem cells from healthy people fibroblast (n=2). A total of 21 samples were analyzed : 9 NSC derived from iPSC LCA and 12 NSC derived from wild-type iPSC.
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Contributor(s) |
Lustremant C, de la Grange P, Monville C |
Citation(s) |
23663011 |
Submission date |
Jan 30, 2013 |
Last update date |
Feb 18, 2019 |
Contact name |
Pierre de la Grange |
E-mail(s) |
pierre.delagrange@genosplice.com
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Organization name |
GenoSplice technology
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Department |
Paris Biotech Santé
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Street address |
29, rue du Faubourg Saint-Jacques
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City |
Paris |
ZIP/Postal code |
75014 |
Country |
France |
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Platforms (1) |
GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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Samples (21)
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GSM1074467 |
hiPSC GM03852cl1-derived Neural Stem Cells at passage 7, biological rep1 |
GSM1074468 |
hiPSC GM03852cl1-derived Neural Stem Cells at passage 7, biological rep2 |
GSM1074469 |
hiPSC GM03852cl1-derived Neural Stem Cells at passage 7, biological rep3 |
GSM1074470 |
hiPSC GM05300cl4-derived Neural Stem Cells at passage 7, biological rep1 |
GSM1074471 |
hiPSC GM05300cl4-derived Neural Stem Cells at passage 7, biological rep2 |
GSM1074472 |
hiPSC GM05300cl4-derived Neural Stem Cells at passage 7, biological rep3 |
GSM1074473 |
hiPSC GM05300cl5-derived Neural Stem Cells at passage 7, biological rep1 |
GSM1074474 |
hiPSC GM05300cl5-derived Neural Stem Cells at passage 7, biological rep2 |
GSM1074475 |
hiPSC GM05300cl5-derived Neural Stem Cells at passage 7, biological rep3 |
GSM1074476 |
hiPSC 46034Acl1-derived Neural Stem Cells at passage 7, biological rep1 |
GSM1074477 |
hiPSC 46034Acl1-derived Neural Stem Cells at passage 7, biological rep2 |
GSM1074478 |
hiPSC 46034Acl1-derived Neural Stem Cells at passage 7, biological rep3 |
GSM1074479 |
hiPSC 4603cl27-derived Neural Stem Cells at passage 7, biological rep1 |
GSM1074480 |
hiPSC 4603cl27-derived Neural Stem Cells at passage 7, biological rep2 |
GSM1074481 |
hiPSC 4603cl27-derived Neural Stem Cells at passage 7, biological rep3 |
GSM1074482 |
hiPSC 4603cl28-derived Neural Stem Cells at passage 7, biological rep1 |
GSM1074483 |
hiPSC 4603cl28-derived Neural Stem Cells at passage 7, biological rep2 |
GSM1074484 |
hiPSC 4603cl28-derived Neural Stem Cells at passage 7, biological rep3 |
GSM1074485 |
hiPSC FSc03-derived Neural Stem Cells at passage 7, biological rep1 |
GSM1074486 |
hiPSC FSc03-derived Neural Stem Cells at passage 7, biological rep2 |
GSM1074487 |
hiPSC FSc03-derived Neural Stem Cells at passage 7, biological rep3 |
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This SubSeries is part of SuperSeries: |
GSE43946 |
Human induced pluripotent stem cells reveal early developmental molecular correlates with a probable Leber congenital amaurosis type I |
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Relations |
BioProject |
PRJNA188200 |