|
| Status |
Public on Mar 28, 2013 |
| Title |
Integration of Metabolic and Gene Regulatory Networks Modulates The C. elegans Dietary Response |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of wildtype C. elegans (N2) and pcca-1(ok2282) and metr-1(ok521) mutants fed Comamonas DA1877
Expression profiles are tailored according to dietary input. However, the networks that control dietary responses remain largely uncharacterized. Here, we combine forward and reverse genetic screens to delineate a network of 184 genes that affect the C. elegans dietary response to Comamonas DA1877 bacteria. We find that perturbation of a mitochondrial network comprised of enzymes involved in amino acid metabolism and the TCA cycle affects the dietary response. In humans, mutations in the corresponding genes cause inborn diseases of amino acid metabolism, most of which are treated by dietary intervention. We identify several TFs that mediate the changes in gene expression upon metabolic network perturbations. Altogether, our findings unveil a transcriptional response system that is poised to sense dietary cues and metabolic imbalances, illustrating extensive communication between metabolic networks in the mitochondria and gene regulatory networks in the nucleus. Expression profiles are tailored according to dietary input. However, the networks that control dietary responses remain largely uncharacterized. Here, we combine forward and reverse genetic screens to delineate a network of 184 genes that affect the C. elegans dietary response to Comamonas DA1877 bacteria. We find that perturbation of a mitochondrial network comprised of enzymes involved in amino acid metabolism and the TCA cycle affects the dietary response. In humans, mutations in the corresponding genes cause inborn diseases of amino acid metabolism, most of which are treated by dietary intervention. We identify several TFs that mediate the changes in gene expression upon metabolic network perturbations. Altogether, our findings unveil a transcriptional response system that is poised to sense dietary cues and metabolic imbalances, illustrating extensive communication between metabolic networks in the mitochondria and gene regulatory networks in the nucleus.
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| |
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| Overall design |
3 biological replicates for each condition, wild type is reference sample
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| |
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| Contributor(s) |
Watson E, MacNeil LT, Arda HE, Zhu LJ, Walhout AJ |
| Citation(s) |
23540702 |
| Submission date |
Jan 31, 2013 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Lesley T MacNeil |
| E-mail(s) |
lesley.macneil@umassmed.edu
|
| Organization name |
UMass Medical School
|
| Department |
Program in Systems Biology
|
| Lab |
Walhout
|
| Street address |
368 Plantation St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
|
| Samples (9)
|
| GSM1074920 |
N2 gravid adult fed Comamonas DA1877 rep 1 |
| GSM1074921 |
N2 gravid adult fed Comamonas DA1877 rep 2 |
| GSM1074922 |
N2 gravid adult fed Comamonas DA1877 rep 3 |
| GSM1074923 |
metr-1(ok521) fed Comamonas DA1877 rep 1 |
| GSM1074924 |
metr-1(ok521) fed Comamonas DA1877 rep 2 |
| GSM1074925 |
metr-1(ok521) fed Comamonas DA1877 rep 3 |
| GSM1074926 |
pcca-1(ok2282) fed Comamonas DA1877 rep1 |
| GSM1074927 |
pcca-1(ok2282) fed Comamonas DA1877 rep2 |
| GSM1074928 |
pcca-1(ok2282) fed Comamonas DA1877 rep3 |
|
| Relations |
| BioProject |
PRJNA188221 |
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