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| Status |
Public on Mar 31, 2013 |
| Title |
Upregulation of ERBB3 by FOXD3, an adaptive response to RAF and MEK inhibitors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To understand the transcriptional impact of FOXD3 in melanoma cells, we utilized a microarray approach. We collected RNA from three unrelated mutant B-RAF melanoma cell lines (WM115, WM793, and A375) that were engineered to inducibly express FOXD3 or the control gene, β-galactosidase (LacZ), after 5 days of transgene induction. This time point was chosen based on maximal phenotypic changes previously observed. Comparison of gene signatures between the 3 cell lines produced approximately 2,600 common genes differentially regulated by FOXD3-expressing cells compared to the LacZ controls.
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| Overall design |
Three unrelated mutant B-RAF melanoma cell lines (WM115, WM793, and A375) were induced to express FOXD3 and compared against the same cell lines expressing the control gene, β-galactosidase (LacZ).
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| Contributor(s) |
Abel EV, Ertel A, Fortina P, Aplin AE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 31, 2013 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Adam Ertel |
| Organization name |
Thomas Jefferson University
|
| Department |
Cancer Biology
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| Lab |
BLSB 1009
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| Street address |
233 S 10TH ST
|
| City |
PHILADELPHIA |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA188229 |
