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| Status |
Public on Feb 06, 2013 |
| Title |
Xi-reactivation in female human induced pluripotent stem cells (hiPSCs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Female human induced pluripotent stem cell (hiPSC) lines exhibit considerable variability in X-inactivation status. Some lines maintain one transcriptionally active X chromosome (Xa) and one inactive X (Xi) from donor cells. However, hiPSC lines that have two Xas are infrequently produced. We show here Xinactivation status in female hiPSC lines depends on derivation conditions. hiPSC lines generated using the Kyoto method, which employs leukemia inhibitory factor (LIF)-expressing SNL feeders, frequently had two Xas. Lines derived on other feeders maintained an Xi. In addition, there appears to be a window in which SNL feeders promote Xi-reactivation. Upon differentiation, Xa/Xa hiPSCs silenced one X. The efficient production of Xa/Xa hiPSC lines provides unprecedented opportunities to understand human X-reactivation and inactivation.
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| Overall design |
Gene expression patterns were compared between several human embryonic stem cell (hESC) and hiPSC lines. Gene expression ratios between genes on X and those on autosomes were calculated from each cell lines.
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| Contributor(s) |
Yamanaka S, Panning B, Takahashi K, Narita M |
| Citation missing |
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| Submission date |
Feb 04, 2013 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Kiichiro Tomoda |
| E-mail(s) |
ktomoda@gladstone.ucsf.edu
|
| Phone |
4157342821
|
| Organization name |
The Gladstone Institutes, UCSF
|
| Street address |
1650 Owens Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (2) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE44066 |
Analyses of X-inactivation status in female human induced pluripotent stem cells |
|
| Relations |
| BioProject |
PRJNA188555 |
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