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Status |
Public on Jul 24, 2013 |
Title |
Human glioma cell line T98G: BAP-PCBP2 RIP vs. BAP-GFP RIP (Control) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
RIP-Chip analysis of PCBP2 and identification of preferentially associated mRNAs. T98G cells were transfected transiently with BAP-tagged constructs. BAP-tagged proteins were biotinylated in vivo by the co-transfected hBirA enzyme. RNPs were recovered via precipitation with Steptavidin-sepharose beads. Finally, RNAs were purified and analyzed on microarrays. BAP-GFP as control was used in three independent sets of experiments.
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Overall design |
Two-condition experiment, BAP-PCBP2 vs. BAP-GFP cells. Biological replicates: 3 BAP-PCBP2 replicates, 3 BAP-GFP (Control) replicates
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Contributor(s) |
Han W |
Citation(s) |
23585479 |
Submission date |
Feb 04, 2013 |
Last update date |
Jan 23, 2019 |
Contact name |
zhong xiao peng |
Organization name |
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
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Street address |
5 Dong Dan San Tiao
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City |
Beijing |
ZIP/Postal code |
100005 |
Country |
China |
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Platforms (1) |
GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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Samples (3) |
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Relations |
BioProject |
PRJNA188445 |