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| Status |
Public on Feb 07, 2013 |
| Title |
Cardiac phase-targeted dynamic load on left ventricle differentially regulates phase-sensitive gene expressions and pathway activation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Each heart was assigned to one of the following three conditions: phasic systolic overload (SO), phasic diastolic overload (DO), and no loading throughout the cardiac cycle (control; CN). We created SO by isovolumic contraction (peak systolic LVP > 170 mmHg) at a constant diastolic volume with end-diastolic pressure (EDP) equals 0 mmHg. For DO, we increased LVV in diastole so that EDP reached 40 mmHg, and unloaded quickly in systole to minimize LVP generation. At the end of 3 hours, the myocardium from each heart was collected for subsequent analyses.
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| Overall design |
rat heart subjected to phasic systolic overload and diastolic overload
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| |
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| Contributor(s) |
Onitsuka K, Ide T, Arai S, Hata Y, Murayama Y, Hosokawa K, Sakamoto T, Tobushi T, Sakamoto K, Fujino T, Sunagawa K |
| Citation(s) |
24004468 |
| Submission date |
Feb 06, 2013 |
| Last update date |
Jul 29, 2014 |
| Contact name |
tomomi ide |
| E-mail(s) |
tomomi_i@cardiol.med.kyushu-u.ac.jp
|
| Phone |
+81-92-642-5360
|
| Fax |
+81-92-642-5374
|
| URL |
http://www.med.kyushu-u.ac.jp/cardiol/index_english.html
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| Organization name |
KYUSHU UNIVERSITY
|
| Department |
GRADUATE SCHOOL OF MEDICAL SCIENCES
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| Lab |
CARDIOVASCULAR MEDICINE
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL6101 |
Illumina ratRef-12 v1.0 expression beadchip |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA188697 |
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