|
| Status |
Public on May 03, 2013 |
| Title |
Whole genome mRNA analysis of wild-type and Id2-deficient virus-specific CD8+ T cells after influenza infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcription factor Inhibitor of DNA binding 2 (Id2) modulates T cell fate decisions but the molecular mechanism underpinning this regulation is unclear. Here, using whole genome mRNA analysis we show that loss of Id2 programs CD8+ T cells to adopt a memory fate with increased Eomesodermin and Tcf7 expression. Our findings reveal that the Id2-E2A axis orchestrates T cell differentiation through the induction or repression of downstream transcription factors essential for effector and memory T cell differentiation.
|
| |
|
| Overall design |
Wild-type and Id2fl/flLckCre+ DbNP366-specific CD8+ T cells were isolated from the spleen of PR8-primed/HKx31-infected Ly5.2+Id2fl/flLckCre+:Ly5.1+ mixed bone marrow chimeric mice ten days after intranasal influenza infection and analysed by whole genome mRNA analysis. Three biological replicates of each genotype were subjected to microarray analysis.
|
| |
|
| Contributor(s) |
Masson F, Belz GT |
| Citation(s) |
23536629, 24723679 |
| Submission date |
Feb 07, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Moshe Olshansky |
| E-mail(s) |
olshansky@wehi.edu.au
|
| Organization name |
Walter & Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Speed
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA188770 |
Less...
More...