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Series GSE44408 Query DataSets for GSE44408
Status Public on Mar 05, 2013
Title Transcriptomic survey of lymph node-positive vs. - negative ductal breast cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary   Lymph node involvement is a major prognostic variable in breast cancer. Whether the molecular mechanisms that drive breast cancer cells to colonize lymph nodes are shared with their capacity to form distant metastases is yet to be established. In a transcriptomic survey aimed at identifying molecular factors associated with lymph node involvement of ductal breast cancer, we found that luminal differentiation, assessed by the expression of estrogen receptor (ER) and/or progesterone receptor (PR) and GATA3, was only infrequently lost in node-positive primary tumors and in matched lymph node metastases. The transcription factor GATA3 critically determines luminal lineage specification of mammary epithelium and is widely considered a tumor and metastasis suppressor in breast cancer. Strong expression of GATA3 and ER in a majority of primary node-positive ductal breast cancer was corroborated by quantitative RT-PCR and immunohistochemistry in the initial sample set, and by immunohistochemistry in an additional set from 167 patients diagnosed of node-negative and –positive primary infiltrating ductal breast cancer, including 102 samples from loco-regional lymph node metastases matched to their primary tumors, as well as 37 distant metastases. These observations suggest that loss of luminal differentiation is not a major factor driving the ability of breast cancer cells to colonize regional lymph nodes.
 
Overall design The transcriptomic study comprises 16 samples from Lymph node metastasis from infiltrating ductal breast carcinoma, 18 samples from Primary node-positive infiltrating ductal,7 samples from Primary node-negative infiltrating ductal and 3 samples from Unaffected lymph node were included. Their RNA was isolated and prepared for hybridization to human Affymetrix GeneChip arrays.
 
Contributor(s) Fernandez PL, Calvo J, Thomson TM, Sanchez-Cid L, Lozano JJ
Citation(s) 24205108
Submission date Feb 19, 2013
Last update date Dec 06, 2018
Contact name Juanjo Lozano
E-mail(s) juanjo.lozano@ciberehd.org
Organization name CIBEREHD
Department Plataforma de Bioinformatica
Street address C/ Rosselló 153
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (44)
GSM1084655 Unaffected lymp node (A_11G-)
GSM1084656 Unaffected lymp node (A_4G-)
GSM1084657 Unaffected lymp node (A_57G-)
Relations
BioProject PRJNA189786

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Supplementary file Size Download File type/resource
GSE44408_RAW.tar 142.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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