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Series GSE44618 Query DataSets for GSE44618
Status Public on Nov 21, 2013
Title Single Cell RNA-Seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA-seq transcriptome measurements are typically performed by isolating RNA from large numbers of cells in culture or tissues. While highly informative, such experiments mask the variability in gene expression patterns that exists between individual cells. To gain insight into the dynamics of gene expression on the level of single-cells, we have carried out the transcriptomes of single-cells from the GM12878 cell line using RNA-seq.
 
Overall design Single GM12878 cells were picked and RNA-seq libraries were generated using the SMART-seq protocol. We also carried out RNA-seq experiments on pools of 10, 30 and 100 cells, on 100pg and 10ng of total RNA, and on pools of 10 cells that were subsequently split into 10 separate sample and processed as if they were single cells in order to assess technical variation in our experiments.
 
Contributor(s) Wold B, Trout D, Marinov G, Williams B
Citation(s) 24299736
Submission date Feb 25, 2013
Last update date May 15, 2019
Contact name Diane E Trout
Organization name California Institute of Technology
Department Biology
Lab Wold
Street address 1200 E California Blvd M/C 156-29
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (62)
GSM1087856 12515-100mers-GM12878_100A-TopHat-1.4.1-GENCODE-V13
GSM1087857 12516-100mers-GM12878_100B-TopHat-1.4.1-GENCODE-V13
GSM1087858 12517-100mers-GM12878_30A-TopHat-1.4.1-GENCODE-V13
Relations
BioProject PRJNA190661
SRA SRP018838

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44618_RAW.tar 84.1 Mb (http)(custom) TAR (of FPKM_TRACKING, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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