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Series GSE44624 Query DataSets for GSE44624
Status Public on May 30, 2013
Title Glycosylation regulates the stability of c-MYC
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Tumorigenesis is characterised by changes in transcriptional regulation and the androgen receptor (AR) has been identified as a key driver in prostate cancer. In this study, we show that the hexosamine biosynthetic pathway (HBP) genes are overexpressed in clinical prostate cancer and androgen-regulated in cell-lines. HBP senses metabolic status of the cell and produces an essential substrate for O-GlcNAc transferase (OGT), which regulates target proteins via glycosylation. Using immunohistochemistry, we found that OGT is up-regulated in the protein level in prostate cancer (n=1987) and its expression correlates with high Gleason Score, pT and pN stages and biochemical recurrence (for all, p<0.0001). Both a small molecule inhibitor and siRNAs targeting OGT decreased prostate cancer cell growth. Microarray profiling revealed that the principal effects of the OGT inhibitor in prostate cancer cells are on cell cycle progression and DNA replication. We identified MYC as a candidate upstream regulator of these genes and found that OGT inhibitor caused a dose-dependent loss of c-MYC protein but not mRNA in cell lines. Finally, we observed a statistically significant co-expression between c-MYC and OGT in human prostate cancer samples (n=1306, p=0.0012).
 
Overall design Total RNA was extracted and experiment has three biological replicates for each condition, conditions are: 12 hours ST045849, 24 hours ST045849, 12 hours vehicle, 24 hours vehicle, 12 hours siOGT, 24 hours siOGT, 12 hours scrambled, 24 hours scrambled
 
Contributor(s) Itkonen HM, Minner S, Guldvik I, Sandman MJ, Tsourlakis MC, Berge V, Svinland A, Schlomm T, Mills IG
Citation(s) 23720054
Submission date Feb 25, 2013
Last update date Aug 13, 2018
Contact name Ian Mills
E-mail(s) ian.mills@ncmm.uio.no
Organization name University of Oslo
Department Centre for Molecular Medicine Norway
Lab Prostate Cancer Research Group
Street address Gaustadalleen 21
City Oslo
ZIP/Postal code 0349
Country Norway
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (24)
GSM1088015 Vehicle 12 hours_1
GSM1088016 ST045849 12 hours_1
GSM1088017 siOGT 12 hours_1
Relations
BioProject PRJNA190675

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44624_RAW.tar 26.2 Mb (http)(custom) TAR
GSE44624_non-normalized.txt.gz 6.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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