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Series GSE44715 Query DataSets for GSE44715
Status Public on Apr 01, 2013
Title Mast cell expression data from murine aryl hydrocarbon receptor (AhR) null, AhR_d and normal control mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary We found that AhR, a unique chemical sensor, is critical in controlling mast cell differentiation, growth and function in vitro and in vivo. This study seeks to further the understanding of the mechanisms of how AhR control mast cell homeostasis.
Overall design Three different batches of bone marrow derived mast cells (BMMCs) from AhR null, AhR_d and normal control mice were cultured in 30% WEHI-3-conditioned medium. BMMCs were purified by negative selection with MicroBeads (Miltenyi Biotec) after 4 weeks in culture. 99% BMMCs were FcεRI+ c-kit+ CD49b- by flow cytometry analysis. The cells were further rested in cytokine free medium for 4h. Subsequently, total RNA was collected. Gene expression profiling was performed on total RNA from mast cells using Illumina Sentrix® Murine Ref8_v2_R3 BeadChips. Details of the design and the gene signatures found are given in the paper associated with this GEO Series: Yufeng Zhou, Hui-Ying Tung, Ying-Ming Tsai, Shih-Chang Hsu, Hui-Wen Chang, Hirokazu Kawasaki, Hsiao-Chun Tseng, Beverly Plunkett, Peisong Gao, Chih-Hsin Hung, Becky M. Vonakis, and Shau-Ku Huang. “Aryl hydrocarbon receptor controls murine mast cell homeostasis”, Blood 2013 Mar 5 [Epub ahead of print]
Contributor(s) Zhou Y, Tung H, Tsai Y, Hsu S, Chang H, Kawasaki H, Tseng H, Plunkett B, Gao P, Hung C, Vonakis BM, Huang S
Citation(s) 23462117
Submission date Feb 27, 2013
Last update date Jun 14, 2018
Contact name Alan E. Berger
Phone 301-938-3565
Organization name Johns Hopkins University
Department School of Medicine
Lab Division of Allergy and Clinical Immunology
Street address 5501 Hopkins Bayview Circle Room 3B.74C
City Baltimore
State/province MD
ZIP/Postal code 21224
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (9)
GSM1089247 Ad-rep1
GSM1089248 Ad-rep2
GSM1089249 Ad-rep3
BioProject PRJNA191075

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44715_RAW.tar 3.1 Mb (http)(custom) TAR
GSE44715_genes_Fig6g_non-norm_data.txt.gz 2.5 Kb (ftp)(http) TXT
GSE44715_non-normalized_data.txt.gz 1.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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