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| Status |
Public on Oct 22, 2014 |
| Title |
IL4 DCs and monocytes stimulated by 13 human vaccines and LPS for 6hr |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
While dendritic cells (DCs) are known to play a major role in the process of vaccination, the mechanisms by which vaccines induce protective immunity in humans remain elusive. Herein, we used gene microarrays to characterize the transcriptional programs induced over time in human monocyte-derived DCs (moDCs) in vitro in response to influenza H1N1 Brisbane, Salmonella enterica and Staphylococcus aureus. We built a data-driven modular analytical framework focused on 204 pathogen-induced gene clusters. The expression of these modules was analyzed in response to 16 well-defined ligands, targeting TLRs, cytoplasmic PAMP receptors and cytokine receptors. This multi-dimensional framework covers the major biological functions of APC, including the IFN response, inflammation, DC maturation, T cell activation, antigen processing, cell motility and histone regulation. This framework was used to characterize the response of monocytes and moDCs to 14 commercially available vaccines. These vaccines displayed quantitatively and qualitatively distinct modular signatures in monocytes and DCs, in particular Fluzone and Pneumovax, highlighting the functional and phenotypic differences between APC subsets. This modular framework allows the application of systems immunology approaches to study early transcriptional changes in human APC subsets in response to pathogens and vaccines, which might guide the development of improved vaccines.
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| Overall design |
64 samples of IL4 DC, and 64 samples of monocytes stimulated by media (controls, designated 1 and 2 to indicate biological replicates), Menomune (Neisseiria meningitis vaccine, MGL), Fluzone 09-10 (Influenza vaccine, FZ), Havrix (Hepatitis A vaccine, HEPA), Ixiaro (Japanese encephalitis vaccine, JPE), ActHib (Haemophilus influenza vaccine, HIB) and Pneumovax (Pneumococcus vaccine, PVX), Engerix-B (Hepatitis B vaccine, HEPB), Zostavax (Herpes Zoster vaccine, HER), and Gardasil (Human Papilloma virus vaccine, HPV), LPS, Ipol (Polio vaccine, POL), Rabies vaccine (RAB), toxoid-based vaccine (TDAP), Varivax (Varicella vaccine, VAR)
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| Contributor(s) |
Banchereau R, Baldwin N, Athale S, Cepika A, Obermoser G, Xu H, Ohouo M, Berthier I, Snipes L, Wang Y, Harrod C, Oh S, Ramilo O, Banchereau J, Chaussabel D, Paluka K, Pascual V |
| Citation(s) |
25335753 |
| Submission date |
Feb 27, 2013 |
| Last update date |
Mar 16, 2023 |
| Contact name |
Nicole Baldwin |
| E-mail(s) |
Nicole.Baldwin@BSWHealth.org
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| Organization name |
Baylor Research Institute
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| Street address |
3434 Live Oak St
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| City |
Dallas |
| ZIP/Postal code |
75204 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (128)
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| This SubSeries is part of SuperSeries: |
| GSE44722 |
Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines |
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| Relations |
| BioProject |
PRJNA191089 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44721_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
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