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| Status |
Public on May 04, 2013 |
| Title |
Genome-wide maps of CENP-A nucleosomes in three neocentromere-containing cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We used native ChIP-seq of CENP-A-containing particles from normal centromeres on alpha-satellite DNA and three naturally-occurring neocentromeres to test the proposed models for the major form of the fundamental repeating unit of centromeric chromatin. We found that the predominant form of the CENP-A particle at the centromere is an octameric nucleosome with loose terminal DNA. Additionally, we found CENP-A nucleosomes are strongly phased on the 171 bp alpha-satellite monomers of normal centromeres, and also display strong positioning and neocentromeres.
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| |
|
| Overall design |
Comparison of CENP-A and bulk nucleosome DNA lengths and positions in three different human neocentromere-containing cell lines
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| |
|
| Contributor(s) |
Black B |
| Citation(s) |
23644596 |
| Submission date |
Feb 27, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ben Black |
| Organization name |
University of Pennsylvania
|
| Street address |
422 Curie Blvd.
|
| City |
Philadelphia |
| ZIP/Postal code |
19103 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA191094 |
| SRA |
SRP018885 |
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