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Series GSE44765 Query DataSets for GSE44765
Status Public on Oct 20, 2013
Title Global profiling of human hair follicle scalp dermal papilla cells using Affymetrix microarrays
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Dermal papilla cells isolated from the human hair follicle are capable of inducing hair growth in recipient epithelia. However, demonstrating disparity from rodent dermal papilla, human cells lose this inductive competance immediately upon growth in culture under normal growth conditions. We grew dermal papilla cells in hanging drop cultures that are morphologically akin to intact dermal papilla, and found that by enhancing the environment for aggregation, we could restore the inductive capacity of human dermal papilla cells in culture. The underlying genes that regulate the inductive potential of dermal papilla cells is not well understood, and we sought to use global profiling to identify key genes and pathways related to inductive competance within dermal papilla cells.
We used Affymetrix microarrays to profile human dermal papilla cells in both hair inducing, and non-hair inducing states.
 
Overall design Affymetrix microarrays were used to to perform profiling of human dermal papilla cells, both as intact tissues (freshly isolated from scalp), and at several stages in subsequent two dimensional culture; cell explant outgrowths (p0), cells at passage 1 (p1), passage 3 (p3) and passage 5 (p5). RNA was isolated from cultured cells 72 hours after feeding. Cells at passage 3 were also grown in hanging drops to form dermal spheroids, that were used for RNA collection 48 hours after establishment. All experiments were performed using tissue from three biological replicates (#D5, D6, D7),
 
Contributor(s) Higgins CA, Christiano AM
Citation(s) 24145441
Submission date Mar 01, 2013
Last update date Mar 25, 2019
Contact name Claire A Higgins
E-mail(s) ch2609@columbia.edu
Organization name Columbia University
Department Department of Dermatology
Lab Christiano Lab
Street address 1150 St Nicholas Avenue, Russ Berrie 307
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (18)
GSM1090232 Scalp dermal papillae-intact tissue-donor D5
GSM1090233 Scalp dermal papillae-intact tissue-donor D6
GSM1090234 Scalp dermal papillae-intact tissue-donor D7
Relations
BioProject PRJNA191608

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Supplementary file Size Download File type/resource
GSE44765_RAW.tar 84.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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