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Series GSE4479 Query DataSets for GSE4479
Status Public on Jun 01, 2006
Title Expression data from CD4+ splenocytes 6h post-sepsis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Lymphocytes are adversely affected during sepsis. Some CD4+ splenocytes undergo apoptosis while others become Th2 polarized. The molecular determinants of these phenotypic changes are not known. Here we compare the transcriptional response of septic CD4 splenocytes to CD4 splenocytes from sham-manipulated animals 6h after sepsis and identify an early transcriptional component to the septic CD4+ splenocyte phenotype.
We used microarrays to detail the global program of gene expression underlying the sepsis-induced changes in CD4+ splenocyte phenotype.
Keywords: disease state analysis
Overall design CD4+ splenocytes were isolated 6h after the surgical induction of sepsis for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain a homogeneous cell population in order to reduce any effects of cellular heterogeneity on expression profiles. To that end, immunomagnetic negative selection was used to enrich CD4+ splenocyte populations to ~91%. (n=5 biological replicates each CLP and sham)
Contributor(s) McDunn JE, Turnbull IR, Polpitiya AD, Tong A, MacMillan SK, Osborne DF, Hotchkiss RS, Colonna M, Cobb PJ
Citation(s) 16931309
Submission date Mar 15, 2006
Last update date Feb 11, 2019
Contact name Jonathan E McDunn
Phone 314-747-0054
Fax 314-362-7474
Organization name Washington University School of Medicine
Department Surgery
Street address 660 South Euclid Avenue
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM100793 septic CD4+ splenocytes at 6h, biological replicate 1
GSM100794 septic CD4+ splenocytes at 6h, biological replicate 2
GSM100795 septic CD4+ splenocytes at 6h, biological replicate 3
BioProject PRJNA94351

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