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Series GSE44901 Query DataSets for GSE44901
Status Public on Jan 21, 2014
Title Maternal western diet primes susceptibility to hepatic inflamation in adult male mouse offspring
Organism Mus musculus
Experiment type Expression profiling by array
Summary Background & Aims: The influences of the maternal diet during gestation has been suggested to be involved in the development of different aspects of the metabolic syndrome. In our mouse model we characterised the role of maternal western diet in the development of non-alcoholic fatty liver disease (NAFLD) in the offspring.
Methods: Female mice were fed either a western (W) or low-fat control (L) semi-synthetic diet before and during gestation and lactation. At weaning, male offspring were assigned either the W or the L diet, generating four experimental groups: WW, WL, LW and LL offspring. Biochemical, histological and epigenetic indicators were investigated at 29 weeks of age.
Results: Male offspring exposed to prenatal western style diet and to a post-weaning W diet (WW) showed hepatomegaly combined with increased hepatic cholesterol and triglycerides accumulation, compared to LW offspring. This was associated with up-regulation of de novo lipid synthesis and dysregulation of beta oxidation and lipid storage. Elevated hepatic transaminases and increased expression of Tnfa, Cd11, Mcp1 and Tgfb underpin the severity of liver injury. Histological analysis supported the presence of steatohepatitis in the WW offspring. In addition alterations in DNA methylation in key metabolic genes (Ppara, Insig, Fasn) were detected.
Conclusion: Maternal dietary fat intake during critical developmental phases programs susceptibility to liver disease in mouse offspring. This was mediated by shifts in lipid metabolism and inflammatory response. Long lasting epigenetic changes may underlie this dysregulation
Overall design 4 groups of 6 male mouse were analysed , 1 experimental and 1 biological outlier was excluded , so n=6,5,5,6 in the 4 groups (LL,LW,WL,WW)
Contributor(s) Pruis MG, Lendvai A, Bloks VW, Zwier MV, Baller JF, Bruin de A, Groen AK, Plosch T
Citation(s) 24224789
Submission date Mar 05, 2013
Last update date Jan 16, 2019
Contact name vincent bloks
Organization name UMCG
Department Pediatrics
Lab Pediatrics
Street address Hanzeplein1
City Groningen
ZIP/Postal code 9713GZ
Country Netherlands
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (22)
GSM1093599 LW1
GSM1093600 WW1
GSM1093601 LW2
BioProject PRJNA192220

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE44901_RAW.tar 15.8 Mb (http)(custom) TAR
GSE44901_non-normalized.txt.gz 4.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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