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Series GSE44933 Query DataSets for GSE44933
Status Public on Apr 11, 2013
Title Type 2 innate signals regulate functionality of fibro/adipogenic progenitors to facilitate muscle regeneration
Organism Mus musculus
Experiment type Expression profiling by array
Summary In vertebrates, activation of innate immunity is an early response to injury, implicating it in the regenerative process. However, the mechanisms by which innate signals might regulate stem cell functionality are unknown. Here we demonstrate that type 2 innate immunity is required for regeneration of skeletal muscle after injury. Muscle damage results in rapid recruitment of eosinophils, which secrete IL-4 to activate the regenerative actions of muscle resident fibro/adipocyte progenitors (FAPs). In FAPs, IL-4/IL-13 signaling serves as a key switch to control their fate and functions. Activation of IL-4/IL-13 signaling promotes proliferation of FAPs to support myogenesis, while inhibiting their differentiation into adipocytes. Surprisingly, type 2 cytokine signaling is also required in FAPs, but not myeloid cells, for rapid clearance of necrotic debris, a process that is necessary for timely and complete regeneration of tissues.
 
Overall design Fibroadipocyte progenitors were isolated from wild-type and IL-4Ra knockout (IL-4Ra -/-) mice and were treated with vehicle or IL4 stimulation in culture prior to microarray analysis. 4 biological replicates of each condition were performed.
 
Contributor(s) Deo RC, Heredia JE, Mukundan L, Chen FM, Mueller AA, Locksley RM, Rando TA, Chawla A
Citation(s) 23582327
Submission date Mar 06, 2013
Last update date Jun 14, 2018
Contact name Rahul C Deo
E-mail(s) rahul.c.deo@gmail.com
Organization name University of California San Francisco
Department Cardiovascular Research Institute
Lab Deo
Street address 555 Mission Bay Blvd. South
City SAN FRANCISCO
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM1090112 KO1
GSM1090113 KO2
GSM1090114 KO3
Relations
BioProject PRJNA192620

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44933_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data included within Sample table
Processed data included within Sample table

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