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Series GSE44954 Query DataSets for GSE44954
Status Public on Jun 14, 2013
Title Gene Expression in Bone-marrow derived Dendritic Cells stimulated with H. pylori lysate vs. E. coli lysate
Organism Mus musculus
Experiment type Expression profiling by array
Summary Gene Expression changes in BMDCs stimulated with H. pylori vs. E. coli. The hypothesis tested was that the gene expression profile in BMDCs stimulated with H. pylori lysate will be less inflammatory than BMDCs stimulated with E. coli.
 
Overall design BMDCs were generated from bone marrow of wild type female C57BL/6 mice using 100ng/mL Flt3L. DCs were harvested at day 8 and stimulated with either media alone, H. pylori antigen lysate or E. coli lysate. At 24 hours, the cells were harvested and RNA was isolated for microarray analysis.
 
Contributor(s) Blanchard T, Shiu J, Sun Y
Citation(s) 23776703
Submission date Mar 07, 2013
Last update date Jun 14, 2018
Contact name Jessica Shiu
E-mail(s) jessica.shiu@som.umaryland.edu
Organization name University of Maryland School of Medicine
Department Pediatrics
Lab Blanchard
Street address 655 W Baltimore St
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1094369 BMDCs- unstimulated 1
GSM1094370 BMDCs- unstimulated 2
GSM1094371 BMDCs- Escherichia coli Ag lysate stimulated 1
Relations
BioProject PRJNA192615

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44954_RAW.tar 3.1 Mb (http)(custom) TAR
GSE44954_non-normalized.txt.gz 883.3 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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