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| Status |
Public on Mar 09, 2013 |
| Title |
Effects of group III phospholipase A2 deficiency in mast cells on fibroblast-directed maturation of mast cells in vitro |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Microenvironment-based alterations in mast cell phenotypes influence the susceptibility to anaphylaxis, yet the mechanisms underlying proper maturation of mast cells toward an anaphylaxis-sensitive phenotype are incompletely understood. Here we report that PLA2G3, a mammalian homolog of anaphylactic bee venom phospholipase A2, regulates this process. PLA2G3 secreted from mast cells is coupled with fibroblastic lipocalin-type PGD2 synthase (L-PGDS) to provide PGD2, which facilitates mast cell maturation via PGD2 receptor (DP1). Mice lacking PLA2G3, L-PGDS or DP1, mast cell-deficient mice reconstituted with PLA2G3- or DP1-null mast cells, or mast cells cocultured with L-PGDS-ablated fibroblasts exhibited impaired mast cell maturation and anaphylaxis. Thus, we describe a lipid-driven PLA2G3-L-PGDS-DP1 loop that drives mast cell maturation.
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| Overall design |
Model, in vitro mast cell maturation; Cells, bone marrow-derived mast cells (BMMCs) cocultured with Swiss 3T3 fibroblasts; Condition, 4 condition experiments: BMMCs cocultured with (mature) versus without (immature) fibroblasts, Pla2g3 knockout (–/–) versus Pla2g3 wild-type (+/+); Samples, 3 replicates of Pla2g3+/+ BMMCs, 4 replicates of Pla2g3–/– BMMCs, 4 replicates of Pla2g3+/+ cocultuzred BMMCs, 3 replicates of Pla2g3–/– cocultured BMMCs
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| Web link |
https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/pubmed/23624557
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| Contributor(s) |
Taketomi Y, Nishito Y, Murakami M |
| Citation(s) |
23624557 |
| Submission date |
Mar 08, 2013 |
| Last update date |
Jan 13, 2024 |
| Contact name |
Yoshitaka Taketomi |
| Organization name |
The University of Tokyo
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| Department |
CDBIM, Graduate School of Medicine,
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| Lab |
Laboratory of Microenvironmental Metabolic Health Science
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| Street address |
7-3-1 Hongo, Bunkyo-ku,
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| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA192684 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44980_RAW.tar |
124.9 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE44980_Significant.txt.gz |
1.0 Kb |
(ftp)(http) |
TXT |
| GSE44980_annotated_data.txt.gz |
2.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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