|
| Status |
Public on Sep 07, 2013 |
| Title |
Gene Regulation in Bob1-/- and miR-146a-/- Murine Pre-B Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
5d IL-7-supported bone marrow cultures from C57BL/6 mice deficient for either the transcription factor Bob1 or microRNA-146a
Micro-RNAs (miRNAs) are required for many processes during hematopoietic development and activation, including B-cell differentiation. The lymphocyte-specific transcription factor Bob1 is critical for the proper development and function of B cells. Bob1-/- mice are immunodeficient and lack germinal centers. Microarray expression analysis of Bob1-deficient B cells shows numerous up-regulated genes, suggesting that Bob1 may affect targets indirectly via the control of miRNAs. Using a gain-of-function/loss-of-function approach in WEHI-231 and primary pro-B and pre-B cells, we identified miR-146a as a direct target of Bob1. Ttranscriptome-wide analysis of miR-146a-/- B cells shows a number of misregulated genes involved in immune regulation, while analysis of Bob1-/- cells shows increased expression of cell cycle-related genes. These results support previous evidence that Bob1 suppresses proliferation, a previously underappreciated role for Bob1 that is in line with the cancer-prone phenotype of mir-146a-/- mice.
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| Overall design |
8 samples in total; 2 biological duplicates each of Bob1wt, Bob1KO, wild-type C57BL/6Ncrl, and mir-146aKO
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| |
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| Contributor(s) |
Lindner JM |
| Citation(s) |
24719463 |
| Submission date |
Mar 08, 2013 |
| Last update date |
Jan 12, 2017 |
| Contact name |
John Lindner |
| E-mail(s) |
lindner@ie-freiburg.mpg.de
|
| Organization name |
MPI-IE Freiburg
|
| Department |
Reth
|
| Lab |
Nielsen
|
| Street address |
Stübeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA192709 |
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